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Series GSE159588 Query DataSets for GSE159588
Status Public on Mar 11, 2024
Title Molecular analysis of the subaortic hematopoietic stem cell niche [E10.5_1]
Organism Mus musculus
Experiment type Expression profiling by array
Summary Hematopoietic stem cells (HSCs) are formed in the Aorta-Gonad-Mesobephros (AGM) region of mouse midgestation embryos. This process is tightly regulated in time and space by surrounding cells inculding mesenchymal cells located underneath the dorsal aorta.
We combined laser microdissection and microarrays to isolate the dorsal and ventral aortic tissues at three developmental stages and to explore their gene expression profiling.
Overall design The dorsal and ventral aortic tissues (located in between the notochord and the roof of the dorsal aorta and underneath the dorsal aorta, respectively) were isolated by laser microdissection in the mouse embryo at three stages of AGM hematopoiesis (E10.5, E11.5 and E12.5). Microdissected tissues were collected on appropriate caps and then proceeded for RNA extraction using Acturus Picopure RNA extraction kit and Affymetrix microarrays mouse Gene 2.0 ST.
Contributor(s) Durand C, Charbord P, Jaffredo T
Citation(s) 38451068
Submission date Oct 19, 2020
Last update date Jun 18, 2024
Contact name Charles Durand
Phone +33 1 44 27 22 84
Organization name Sorbonne University
Department Laboratory of Developmental Biology
Lab CNRS UMR7622
Street address 9, quai saint-Bernard
City Paris
ZIP/Postal code 75005
Country France
Platforms (1)
GPL23092 [MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array (mogene20st_Mm_ENTREZG_21.0.0)
Samples (6)
GSM4832851 Dorsal tissue at E10.5, biological rep 1
GSM4832852 Dorsal tissue at E10.5, biological rep 2
GSM4832853 Dorsal tissue at E10.5, biological rep 3
This SubSeries is part of SuperSeries:
GSE159592 Molecular analysis of the subaortic hematopoietic stem cell niche
BioProject PRJNA670012

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE159588_RAW.tar 52.8 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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