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Series GSE159679 Query DataSets for GSE159679
Status Public on Oct 21, 2020
Title Spontaneous Cell Fusions as a Mechanism of Parasexual Recombination in Tumor Cell Populations. [1]
Organism Homo sapiens
Experiment type SNP genotyping by SNP array
Summary Initiation and progression of cancers reflect the underlying process of somatic evolution, which follows a Darwinian logic, i.e., diversification of heritable phenotypes provides a substrate for natural selection, resulting in the outgrowth of the most fit subpopulations. Although somatic evolution can tap into multiple sources of diversification, it is assumed to lack access to (para)sexual recombination – a key diversification mechanism throughout all strata of life. Based on observations of spontaneous fusions involving cancer cells, reported genetic instability of polypoid cells, and precedence of fusion-mediated parasexual recombination in fungi, we asked whether cell fusions could serve as a source of parasexual recombination in cancer cell populations. Using differentially labelled tumor cells, we found evidence of low-frequency, spontaneous cell fusions between carcinoma cells in multiple cell line models of breast cancer both in vitro and in vivo. While some hybrids remained polyploid, many displayed partial ploidy reduction, generating diverse progeny with heterogeneous inheritance of parental alleles, indicative of partial recombination. Hybrid cells also displayed elevated levels of phenotypic plasticity, which may further amplify the impact of cell fusions on the diversification of phenotypic traits. Using mathematical modeling, we demonstrated that the observed rates of spontaneous somatic cell fusions may enable populations of tumor cells to amplify clonal heterogeneity, thus facilitating the exploration of larger areas of the adaptive landscape, relative to strictly asexual populations, which may substantially accelerate a tumor’s ability to adapt to new selective pressures.
 
Overall design Illumina CytoNP arrays were performed according to the manufacturer's protocol. DNA was extracted from frosen cell pellet using Quiagen DNAeasy kit. 12 samples were analysed: 8 subclones of hybrid between SUM159 and MDA231 cell lines, 2 bulk samples from passage 3 ans passage 8 of hybrid SUM159-MDA231 cells. Parental cell lines: MDA231, SUM159. Cell were grown in correcponding media before collecting the samples, no treatment was applied.
 
Contributor(s) Miroshnychenko D
Citation(s) 33462489
Submission date Oct 20, 2020
Last update date Jan 20, 2021
Contact name Daria Miroshnychenko
E-mail(s) daria.miroshnychenko@moffitt.org
Organization name Moffitt Cancer Center
Department Cancer Physiology
Lab Marusyk Lab
Street address 12902 USF Magnolia Drive
City TAMPA
State/province FL
ZIP/Postal code 33612
Country USA
 
Platforms (1)
GPL29257 Illumina HumanCytoSNP-12v2-1_L1
Samples (12)
GSM4837580 A1
GSM4837581 A2
GSM4837582 A3
This SubSeries is part of SuperSeries:
GSE159681 Spontaneous Cell Fusions as a Mechanism of Parasexual Recombination in Tumor Cell Populations
Relations
BioProject PRJNA670184

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE159679_Illumina2018_Matrix_processed_Miroshnychenko.txt.gz 71.0 Mb (ftp)(http) TXT
GSE159679_Illumina2018_Matrix_signal_intensities_Miroshnychenko.txt.gz 51.2 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

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