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Series GSE16007 Query DataSets for GSE16007
Status Public on Jan 01, 2010
Title miR-140 deficiency effect on the chondrocytes
Organism Mus musculus
Experiment type Expression profiling by array
Summary Analysis of mouse chondrocytes lacking the microRNA-140. MicroRNAs are genomically encoded small RNAs to regulate the gene expression. miR-140 shows high expression in cartilage. Results provide insight into the molecular mechanisms underlying miR-140 function in chondrocytes.

Keywords: Expression profiling by array
 
Overall design DNA microarray analysis was performed using Affymetrix mouse genome 430 2.0 array. RNA samples collected from cultured rib chondrocytes of wild-type and miR-140-/- to identify potential mRNA targets of miR-140. 2 µg of total RNA was reverse transcribed with SuperScript II and second strand cDNA was synthesized. Biotinylated antisense cRNAs was amplified and transcribed using the GeneChip expression 3’-Amplification reagent (Affymetrix). Finally, 10 µg of cRNAs were fragmented and hybridized to GeneChip (R) Mouse Genome 430 2.0 array (Affymetrix). Microarray data were summarized by Robust Multichip Average (RMA) method and statistical analysis was performed using NIA Array Analysis (http://Igusun.grc.nia.nih.gov/ANOVA/).
 
Contributor(s) Miyaki S, Sato T, Asahara H
Citation(s) 20466812
Submission date May 07, 2009
Last update date Feb 11, 2019
Contact name Hiroshi Asahara
E-mail(s) asahara@nch.go.jp
Phone +81-3-3417-2948
Fax +81-3-3417-2498
Organization name National Research Institute for Child Health and Development
Department Department of Systems BioMedicine
Street address 2-10-1 Okura
City Setagaya
State/province Tokyo
ZIP/Postal code 157-8535
Country Japan
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (2)
GSM400577 rib chondrocyte_cultured_WT
GSM400578 rib chondrocyte_cultured_miR-140 KO
Relations
BioProject PRJNA115429

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE16007_RAW.tar 7.4 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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