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Series GSE160162 Query DataSets for GSE160162
Status Public on Oct 02, 2023
Title Hydrogen sulfide treatment at the late growth stage of Saccharomyces cerevisiae extends chronological lifespan
Organism Saccharomyces cerevisiae
Experiment type Expression profiling by high throughput sequencing
Summary Hydrogen sulfide (H2S), a traditionally known cytotoxic gas, has been recently included in the gasotransmitters family. Previous studies demonstrated that lifelong treatment with a slow H2S releasing donor extends yeast chronological lifespan (CLS), but it is not clear when the action of H2S benefits to CLS during yeast growth. Therefore, we investigated the effects of short H2S treatments at different time during yeast cell growth in the lifespan extension by using NaHS, a fast H2S-releasing donor. We show that short NaHS treatments at 4 days after inoculation extend yeast CLS while NaHS treatments earlier than 3 days after inoculation fail to do so. To reveal the mechanism of different consequences on yeast CLS of NaHS treatments at different times, we analyzed the transcriptome of Saccharomyces cerevisiae strain BY4742 with or without the early and late NaHS treatments. We found that the early and late NaHS treatments had similar effects on the expression of genes involved in pathways which are related to CLS regulation. Follow up qPCR and ROS analyses suggest that altered expression of some antioxidant genes by the early NaHS treatments were not stable enough to benefit CLS. Moreover, transcriptome data also indicated that some genes were regulated differently by the early and late H2S treatment such as genes involved in cell wall integrity. Specifically, we found that the expression of YPK2, a human SGK2 homolog and also a key regulator of the yeast cell wall synthesis, was significantly altered by the late NaHS treatment but not altered by the early NaHS treatment. Finally, the key role of YPK2 in CLS regulation by H2S is revealed by CLS data showing that the late NaHS treatment did not enhance the CLS of a ypk2 knockout mutant. This study sheds light on the molecular mechanism of CLS extension induced by H2S, and for the first time addresses the importance of H2S treatment timing for lifespan extension.
Overall design Four samples, three replicates each. Names are Early_control, Early_NaHS_treatment, Late_control and Late_NaHS_treatment.
Contributor(s) Ali Shah A, Liu B, Wang W, Liu K
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Submission date Oct 26, 2020
Last update date Oct 02, 2023
Contact name Arman Ali Shah
Phone 008613032865903
Organization name Sichuan University
Street address Wangjiang road 29, Key Laboratory of Bio-resources and Eco-environment of the Ministry of Education, College of Life Sciences, Sichuan University, Chengdu
City Chengdu
State/province Sichuan
ZIP/Postal code 610064
Country China
Platforms (1)
GPL27812 Illumina NovaSeq 6000 (Saccharomyces cerevisiae)
Samples (12)
GSM4861683 Early_control, rep1
GSM4861684 Early_control, rep2
GSM4861685 Early_control, rep3
BioProject PRJNA672161
SRA SRP288909

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Supplementary file Size Download File type/resource
GSE160162_Early_control_vs_Early_NaHS_treatment.txt.gz 118.3 Kb (ftp)(http) TXT
GSE160162_Late_control_vs_late_NaHS_treatment.txt.gz 139.6 Kb (ftp)(http) TXT
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