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| Status |
Public on Apr 14, 2021 |
| Title |
MLL3 suppresses tumorigenesis through regulating TNS3 enhancer activity [ChIP-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
MLL3 is a histone H3K4 methyltransferase which is frequently mutated in cancer, but the underlying molecular mechanisms remain elusive. Here, we found that MLL3 knockout by CRISPR/sgRNA did not elevate proliferation rate of cancer cells, but significantly enhanced cell migration. Through RNA-Seq and ChIP-Seq approaches, we identified TNS3 as the potential target gene for MLL3. MLL3 depletion caused down regulation of H3K4me1 and H3K27ac on an enhancer ~ 8 kb ahead of TNS3. 3C assay indicated the identified enhancer interacts with TNS3 promoter, and repression of enhancer with dCas9-KRAB system impaired TNS3 expression. Exogenous expression of TNS3 in MLL3 deficient cells completely blocked the enhanced cell migration phenotype. Taken together, our study revealed a novel mechanism for MLL3 in suppressing cancer, which may provide novel targets for diagnosis or drug development.
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| |
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| Overall design |
Examination of 2 different histone modifications in U2OS cells after MLL3 knockdown.
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| |
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| Contributor(s) |
Zheng J, Wang C, Gao C, Xiao Q, Huang C, Wu M, Li L |
| Citation(s) |
33824309 |
| |
| Submission date |
Oct 26, 2020 |
| Last update date |
Apr 14, 2021 |
| Contact name |
Jun-Yi Zheng |
| Organization name |
Wuhan Univisity
|
| Street address |
No. 299 Of Bayi Road
|
| City |
Wuhan |
| ZIP/Postal code |
430072 |
| Country |
China |
| |
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| Platforms (1) |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE160254 |
MLL3 suppresses tumorigenesis through regulating TNS3 enhancer activity |
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| Relations |
| BioProject |
PRJNA672157 |
| SRA |
SRP288911 |
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