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Series GSE16071 Query DataSets for GSE16071
Status Public on Jun 01, 2009
Title Loss of H3K27 monomethylation leads to DNA-methylation-independent release of gene silencing
Organism Arabidopsis thaliana
Experiment type Methylation profiling by high throughput sequencing
Summary Constitutive heterochromatin in Arabidopsis thaliana is marked by repressive chromatin modifications including DNA methylation, histone 3 dimethylation at lysine 9 (H3K9me2), and monomethylation at lysine 27 (H3K27me1). The enzymes catalyzing DNA methylation and H3K9me2 have been identified and mutations in these proteins lead to the reactivation of silenced heterochromatic elements. The enzymes responsible for heterochromatic H3K27me1, in contrast, remain unknown. Here we show that the divergent SET-domain proteins ARABIDOPSIS TRITHORAX-RELATED PROTEIN5 (ATXR5) and ATXR6 exhibit H3K27 monomethyltransferase activity and double mutants have reduced H3K27me1 in vivo and show partial heterochromatin decondensation. atxr5 atxr6 plants also show transcriptional activation of repressed heterochromatic elements. Interestingly, H3K9me2 and DNA methylation are unaffected in the double mutant. These results indicate that ATXR5 and ATXR6 form a novel class of H3K27 methyltransferases and that H3K27me1 represents a new pathway required for transcriptional repression in Arabidopsis.
 
Overall design Comparison of methylation in wild type and ATXR6/ATXR6 mutants
 
Contributor(s) Michaels SD, Pellegrini M
Citation(s) 19503079
Submission date May 12, 2009
Last update date May 15, 2019
Contact name matteo Pellegrini
E-mail(s) matteop@mcdb.ucla.edu
Phone 310-825-0012
Organization name UCLA
Department MCD Biology
Lab Matteo Pellegrini
Street address 621 Charles Young Drive South
City Los Angeles
State/province CA
ZIP/Postal code 90095
Country USA
 
Platforms (1)
GPL9062 Illumina Genome Analyzer (Arabidopsis thaliana)
Samples (2)
GSM402423 Wild type
GSM402424 atxr5/atxr6 double mutant
Relations
SRA SRP000999
BioProject PRJNA115495

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