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| Status |
Public on Dec 07, 2020 |
| Title |
Area postrema cell types that mediate nausea-associated behaviors |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Nausea, the unpleasant sensation of visceral malaise, remains a mysterious process. The area postrema is implicated in some nausea responses, and contains poorly understood brain-resident sensory neurons that are anatomically privileged to detect bloodborne signals. To investigate nausea mechanisms, we built an area postrema cell atlas through single-nucleus RNA sequencing, revealing a few neuron types. Using mouse genetic tools for cell-specific manipulation, we discovered excitatory neurons that induce nausea-related behaviors, with one neuron type mediating aversion imposed by multiple poisons. Nausea-associated responses were observed to agonists of identified area postrema receptors, and suppressed by targeted cell ablation and/or gene knockout. Anatomical mapping revealed a distributed network of long-range excitatory but not inhibitory projections with subtype-specific patterning. These studies reveal the basic organization of area postrema nausea circuitry, and provide a framework towards understanding and therapeutically controlling nausea.
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| Overall design |
Single-nuclei sequencing of murine area postrema.
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| Contributor(s) |
Liberles SD, Zhang C, Kaye JA, Cai Z, Wang Y, Prescott SL |
| Citation(s) |
33278342 |
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| Submission date |
Nov 05, 2020 |
| Last update date |
Dec 09, 2020 |
| Contact name |
Judith Kaye |
| Organization name |
Harvard University
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| Department |
Cell Biology
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| Lab |
Liberles
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| Street address |
240 Longwood Ave, LHRRB 6th floor
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| City |
Boston |
| State/province |
Massachusetts |
| ZIP/Postal code |
02115 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (2) |
| GSM4886767 |
Single nuclei from the area postrema replicate 1 |
| GSM4886768 |
Single nuclei from the area postrema replicate 2 |
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| Relations |
| BioProject |
PRJNA674867 |
| SRA |
SRP291333 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE160938_RAW.tar |
129.7 Mb |
(http)(custom) |
TAR (of RDS) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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