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Series GSE16105 Query DataSets for GSE16105
Status Public on Jul 15, 2009
Title Trf4p in vivo crosslinking and ribonucleoprotein-immunopurification-chip analysis (X-RIP-Chip)
Organism Saccharomyces cerevisiae
Experiment type Other
Summary In vivo cross-linking and ribonucleoprotein-immunopurification experiments followed by microarray analysis of bound RNAs (X-RIP-chip). Cells expressing recombinant tandem-affinity purification (TAP)-tagged Trf4 protein were cross-linked with formaldehyde, and Trf4-containing ribonucleoprotein complexes were recovered by affinity selection on IgG-coupled beads (see linked protocol). As a control for non-specifically enriched RNAs, the same experiment was done with untagged WT cells and with cells expressing Fpr1-TAP, a peptidyl-prolyl-cis-trans-isomerase not expected to bind RNA.
Set of arrays organized by shared biological context, such as organism, tumors types, processes, etc.
Antigenic peptide used in IP: Protein A derivative

 
Overall design Computed
 
Contributor(s) Gerber A
Citation(s) 19593367
Submission date May 14, 2009
Last update date Mar 21, 2012
Contact name Andre Gerber
E-mail(s) andre.gerber@pharma.ethz.ch
Phone +41 44 6337378
Fax +41 44 6337364
Organization name Swiss Federal Institute of Technology
Department Institute of Pharmaceutical Science
Street address Wolfgang Pauli Str. 10, HCI H396
City ETH Hoenggerberg, Zuerich
ZIP/Postal code ZH
Country Switzerland
 
Platforms (2)
GPL8306 SMD Print_1381 Saccharomyces cerevisiae
GPL8555 SMD Print_1380 Saccharomyces cerevisiae
Samples (6)
GSM402991 Trf4 X-RIP (3)
GSM402992 BY4741 X-RIP
GSM402993 Fpr1 X-RIP (2)
This SubSeries is part of SuperSeries:
GSE16107 trf4, trf5, and trf4-DADA mutant cells gene expression; Trf4p X-RIP-Chip
Relations
BioProject PRJNA122819

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE16105_RAW.tar 2.1 Mb (http)(custom) TAR
Processed data included within Sample table

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