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Status |
Public on Jul 17, 2023 |
Title |
H3K27Ac modification and gene expression in psoriasis |
Organism |
Homo sapiens |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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Summary |
Applying ChIP-seq with anti-acetylation of lysine 27 on Histone 3 (H3K27Ac), we report high-throughput profiling of H3K27Ac in human skin biopsies taken from psoriasis patients, both from the lesion and from adjacent non-lesion skin, and from skin biopsies from healthy match volunteers, in term of age, gender, and the biopsies place in the body. From each group we had 4 samples. There is a clearly different H3K27 acetylation patterns in psoriatic skin compared to uninvolved or healthy volunteer skin. in many of the most over express genes in psoriasis lesion, there is enrichment of H3K27Ac. However, loss of acetylation on H3K27 is not part of the biochemical mechanism by which gene expression is decreased in psoriatic skin. Finally, we show Many of the over express genes in psoriasis lesion, that also were enriched with H3K27Ac harbor a putative GRHL transcription factor binding site.
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Overall design |
ChIP-seq with anti H3K27Ac and mRNA array was applied on skin biopsies from psoriasis patients, both from the lesion and from adjacent non-lesion skin. in parallel to skin biopsies from healthy match volunteers.
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Contributor(s) |
Avni D, Masalha M |
Citation(s) |
34281744 |
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Submission date |
Nov 09, 2020 |
Last update date |
Jul 18, 2023 |
Contact name |
Dror Avni |
Organization name |
Weizmann InstituteSheba Medical Center -Tel Hashomer
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Department |
Department of Medicine C
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Lab |
Laboratory of Molecular Cell Biology
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Street address |
Derech Shiba, 2
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City |
Ramat Gan |
ZIP/Postal code |
52621 |
Country |
Israel |
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Platforms (1) |
GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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Samples (15)
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Relations |
BioProject |
PRJNA675500 |
SRA |
SRP291703 |