NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE161076 Query DataSets for GSE161076
Status Public on Jul 17, 2023
Title H3K27Ac modification and gene expression in psoriasis
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Applying ChIP-seq with anti-acetylation of lysine 27 on Histone 3 (H3K27Ac), we report high-throughput profiling of H3K27Ac in human skin biopsies taken from psoriasis patients, both from the lesion and from adjacent non-lesion skin, and from skin biopsies from healthy match volunteers, in term of age, gender, and the biopsies place in the body. From each group we had 4 samples. There is a clearly different H3K27 acetylation patterns in psoriatic skin compared to uninvolved or healthy volunteer skin. in many of the most over express genes in psoriasis lesion, there is enrichment of H3K27Ac. However, loss of acetylation on H3K27 is not part of the biochemical mechanism by which gene expression is decreased in psoriatic skin. Finally, we show Many of the over express genes in psoriasis lesion, that also were enriched with H3K27Ac harbor a putative GRHL transcription factor binding site.
 
Overall design ChIP-seq with anti H3K27Ac and mRNA array was applied on skin biopsies from psoriasis patients, both from the lesion and from adjacent non-lesion skin. in parallel to skin biopsies from healthy match volunteers.
 
Contributor(s) Avni D, Masalha M
Citation(s) 34281744
Submission date Nov 09, 2020
Last update date Jul 18, 2023
Contact name Dror Avni
Organization name Weizmann InstituteSheba Medical Center -Tel Hashomer
Department Department of Medicine C
Lab Laboratory of Molecular Cell Biology
Street address Derech Shiba, 2
City Ramat Gan
ZIP/Postal code 52621
Country Israel
 
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (15)
GSM4889297 N1
GSM4889298 N2
GSM4889299 N3
Relations
BioProject PRJNA675500
SRA SRP291703

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE161076_RAW.tar 11.7 Mb (http)(custom) TAR (of NARROWPEAK)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap