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Series GSE161310 Query DataSets for GSE161310
Status Public on Sep 30, 2021
Title The histone acetyltransferase HBO1 (KAT7) promotes efficient tip cell sprouting during angiogenesis
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Blood vessel growth and remodelling are essential during embryonic development and disease pathogenesis. The diversity of endothelial cells (ECs) is transcriptionally evident and ECs undergo dynamic changes in gene expression during vessel growth and remodelling.Here, we investigated the role of the histone acetyltransferase HBO1 (KAT7), which is important for activating genes during development and histone H3 lysine 14 acetylation (H3K14ac). Loss of HBO1 and H3K14ac impaired developmental sprouting angiogenesis and reduced pathological EC overgrowth in the retinal endothelium. Single-cell RNA-sequencing of retinal ECs revealed an increased abundance of tip cells in Hbo1 deleted retinas, which lead to EC overcrowding in the retinal sprouting front and prevented efficient tip cell migration. We found that H3K14ac was highly abundant in the endothelial genome in both intra- and intergenic regions suggesting that the role of HBO1 is as a genome organiser that promotes efficient tip cell behaviour necessary for sprouting angiogenesis.
 
Overall design Single cell RNA-seq profiling of P6 retinal endothelial cells from Hbo1 EC-deleted (n = 4) and heterozygous control mice (n = 4)
 
Contributor(s) Grant ZL, Hickey PF, Voss AK, Coultas L
Citation(s) 34550360
Submission date Nov 11, 2020
Last update date Sep 30, 2021
Contact name Peter Hickey
E-mail(s) hickey@wehi.edu.au
Organization name WEHI
Street address 1G Royal Parade
City Parkville
ZIP/Postal code 3052
Country Australia
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (7)
GSM4904700 Plate number LC279
GSM4904701 Plate number LC280
GSM4904702 Plate number LC294
This SubSeries is part of SuperSeries:
GSE161893 The histone acetyltransferase HBO1 (KAT7) promotes efficient tip cell sprouting during normal and pathogenic angiogenesis
Relations
BioProject PRJNA676207
SRA SRP292444

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE161310_ERCC_counts.csv.gz 73.0 Kb (ftp)(http) CSV
GSE161310_gene_counts.csv.gz 9.5 Mb (ftp)(http) CSV
GSE161310_sample_sheet.csv.gz 193.7 Kb (ftp)(http) CSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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