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| Status |
Public on Dec 31, 2009 |
| Title |
Transcriptome analysis of mesothelioma induced by intraperitoneal injections of ferric saccharate in rats |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
Iron plays an important role in oxidative tissue damage and subsequent carcinogenesis. Iron deposition in the peritoneum causes neoplastic changes. Here we used gene expression microarrays to find common genetic alterations in a rat model by repeated intraperitoneal injections of ferric saccharate. Gene expression microarray revealed the expression of uromodulin (Umod) is increased in mesothelioma approximately 250-fold.
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| Overall design |
Carcinogenesis protocol was performed using specific pathogen-free male and female F1 hybrid rats between Fischer344 and Brown-Norway strains. A total of 12 microarrays (Whole Rat Genome Oligo Microarrary) were used for the screening purpose;
The scraped pleural mesothelial cells of 8 week male rat untreated or peri-testicular tissue as control; Induced mesothelioma of epithelioid and sarcomatoid types.
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| Contributor(s) |
Toyokuni S, Hu Q, Akatsuka S |
| Citation(s) |
20065947 |
| |
| Submission date |
May 17, 2009 |
| Last update date |
Sep 13, 2014 |
| Contact name |
Shinya Toyokuni |
| E-mail(s) |
akatsuka@med.nagoya-u.ac.jp
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| Organization name |
Nagoya University
|
| Department |
Pathology
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| Street address |
65 Tsuruma-Cho, Showa-Ku
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| City |
Nagoya |
| State/province |
Aichi |
| ZIP/Postal code |
466-8550 |
| Country |
Japan |
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| Platforms (1) |
| GPL7294 |
Agilent-014879 Whole Rat Genome Microarray 4x44K G4131F (Probe Name version) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA115039 |
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