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Series GSE161818 Query DataSets for GSE161818
Status Public on Sep 15, 2022
Title DNA replication timing directly regulates the frequency of oncogenic chromosomal translocations
Organism Mus musculus
Experiment type Other
Summary Chromosomal translocations result from the joining of DNA double-strand breaks (DSBs) and frequentlycause cancer. However, the steps linking DSB formation to DSB ligation remain undeciphered. Wereport that DNA replication timing (RT) directly regulates lymphomagenicMyctranslocations duringantibody maturation in B cells downstream of DSBs and independently of DSB frequency. Depletion ofminichromosome maintenance complexes alters replication origin activity, decreases translocations,and deregulates global RT. Ablating a single origin atMyccauses an early-to-late RT switch, loss oftranslocations, and reduced proximity with the immunoglobulin heavy chain (Igh) gene, its majortranslocation partner. These phenotypes were reversed by restoring early RT. Disruption of early RT alsoreduced tumorigenic translocations in human leukemic cells. Thus, RT constitutes a general mechanismin translocation biogenesis linking DSB formation to DSB ligation.
Overall design LAM-HTGTS in LacZ and Mcm infected CH12 cells, C13 deltaori and C23 deltaori clones, C13 restored and C2 restored clones using a Myc-bait; LacZ and Mcm infected CH12 cells, C13 deltaori and C13 restored clones using a Irf2bp2-bait
Contributor(s) Peycheva M, Neumann T, Malzl D, Nazarova M, Schoeberl UE, Pavri R
Citation(s) 36108018
Submission date Nov 19, 2020
Last update date Dec 15, 2022
Contact name Tobias Neumann
Organization name IMP
Street address Campus-Vienna-Biocenter 1
City Vienna
ZIP/Postal code 1030
Country Austria
Platforms (1)
GPL24973 MinION (Mus musculus)
Samples (44)
GSM4915155 CH12_LAM-HTGTS_shLacZ_unstimulated
GSM4915156 CH12_LAM-HTGTS_shMcm_unstimulated
GSM4915157 CH12_LAM-HTGTS_shLacZ_rep1
This SubSeries is part of SuperSeries:
GSE161822 DNA replication timing directly regulates the frequency of oncogenic chromosomal translocations
BioProject PRJNA679571
SRA SRP293267

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Supplementary file Size Download File type/resource
GSE161818_HTGTS_Irf2bp2-bait_confident_hotspot_quantification.txt.gz 3.1 Kb (ftp)(http) TXT
GSE161818_HTGTS_Myc-bait_confident_hotspot_quantification.txt.gz 3.6 Kb (ftp)(http) TXT
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Processed data are available on Series record

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