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Series GSE162018 Query DataSets for GSE162018
Status Public on Nov 23, 2023
Title Next generation sequencing of long non-coding RNAs in diabetes mellitus rats after sleeve gastrectomy
Organism Rattus norvegicus
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary Sleeve gastrectomy (SG) could improve diabetes mellitus dramatically. However, the mechanisms remain largely undetermined. With the advancement of in-depth bioinformatic analysis, non-coding RNAs especially long non-coding RNAs(lncRNAs), have been implicated in many biological processes. To explore whether lncRNAs mediate the amelioration of diabetes mellitus condition after SG, we subjected liver samples of diabetic rats after SG and shame operations for RNA sequencing. Further, interaction network between lncRNAs and mRNAs/microRNAs(miRNAs) were constructed to infer the function of lncRNAs in diabetic remission, followed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses. Through RNA sequencing, we identified more than 9000 lncRNAs with a dramatically differentially expressed profile of 147 lncRNAs. Some of these specific lncRNAs were only expressed in diabetic rats after SG, which may be promising biological markers of diabetic remission and SG prognosis. Through co-analysis between lncRNAs and mRNAs, 20 potential target genes of lncRNAs were screened out. Further bioinformatic analysis indicated the differentially expressed lncRNAs harbored various miRNAs binding sites and three representative lncRNA-miRNA networks of lnc50 and lnc136 were constructed. The KEGG analysis revealed that differential expressed lncRNAs were mainly related to mitogen-activated protein kinase (MAPK) pathway. These results demonstrated that SG dramatically altered expression profile of lncRNAs on diabetes mellitus, and altered lncRNAs expression may contribute to the improvement on diabetic remission through MAPK pathway by interaction with mRNA/miRNA. Our findings provide valuable resources for molecular mechanism of diabetic remission after SG and is benefit for development of lncRNAs-based diagnostic and therapeutic interventions of diabetes mellitus.
Overall design Expression profile of long non-coding RNAs in diabetes mellitus rats after sleeve gastrectomy
Contributor(s) Li L, Hu S
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Submission date Nov 23, 2020
Last update date Nov 23, 2023
Contact name Linchuan Li
Organization name Shandong University
Street address 16766# Jingshi Road
City Jinan
State/province Shandong
ZIP/Postal code 250000
Country China
Platforms (1)
GPL22396 Illumina HiSeq 4000 (Rattus norvegicus)
Samples (10)
GSM4929793 SG1 lnc
GSM4929794 SG2 lnc
GSM4929795 SG3 lnc
This SubSeries is part of SuperSeries:
GSE162022 Next generation sequencing of circular and lnc RNAs in diabetes mellitus rats after sleeve gastrectomy
BioProject PRJNA680323
SRA SRP293888

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Supplementary file Size Download File type/resource
GSE162018_5_isoforms_fpkm_expression.txt.gz 7.1 Mb (ftp)(http) TXT
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