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Series GSE162185 Query DataSets for GSE162185
Status Public on Jun 05, 2021
Title Next-Generation Sequencing Transcriptome Quantitative Analysis of Hearts of Male Fetuses From Obese Female Mice
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Obesity during pregnancy increase the risk for cardiac in the offspring. We used RNA next-generation sequencing analysis to have a snapshot of the heart transcriptome in male offspring exposed to maternal obesity and weaned onto an obesogenic diet.
Overall design Female C57BL/6J mice received either commercial standard (RM1) or a high-fat diet [20% lipids (Special Dietary Services)] plus sweetened condensed milk [55% simple carbohydrates/8% lipids (Nestle, UK)] from weaning. After around 6 weeks on their respective diets, mice were mated with male counterparts and went through a first pregnancy and lactation to ensure their breeding competence. Mice were mated a second time, and first day of pregnancy was marked by the detection of a vaginal plug. On day 18.5 of gestation, pregnant mice were killed by raising CO2 concentrations or cervical dislocation. Fetuses were surgically removed and immediately killed by laying in cold buffer. Fetal heart ventricles were dissected, weighed, snap frozen and stored at −80 °C. Cardiac RNA from male offspring was extracted using a Qiazol/miRNeasy mini kit protocol (Qiagen, Hilden, Germany). Library preparation for mRNA sequencing followed manufacturer protocol of TruSeq RNA Library Preparation kit (Illumina, Cambridge, UK).
Contributor(s) Loche E, Ozanne SE, Wilczynska A, Pantaleao LC
Citation(s) 35025731
Submission date Nov 25, 2020
Last update date Feb 08, 2022
Contact name Lucas Pantaleao
Phone +447842771748
Organization name University of Cambridge
Street address 11A Glisson Road
City Cambridge
ZIP/Postal code CB1 2HA
Country United Kingdom
Platforms (1)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
Samples (8)
GSM4946167 RC65-3A
GSM4946168 RC72-2A
GSM4946169 RC73-2A
BioProject PRJNA680804
SRA SRP294190

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Supplementary file Size Download File type/resource
GSE162185_SLX-9052.C6700ANXX.s_3.contents.csv.gz 261 b (ftp)(http) CSV
GSE162185_raw_count.txt.gz 1.4 Mb (ftp)(http) TXT
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