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Series GSE162273 Query DataSets for GSE162273
Status Public on Feb 21, 2022
Title RNA aptamers specific for transmembrane p24 trafficking protein 6 and Clusterin for the targeted delivery of imaging reagents and RNA therapeutic to human β cells
Organism Homo sapiens
Experiment type Protein profiling by protein array
Summary The ability to detect and target β cells in vivo can drastically refine the way diabetes is studied and treated. By an unsupervised Systematic evolution of ligands by exponential enrichment (SELEX) we identified two RNA aptamers that specifically recognize mouse and human β cells in vitro and in vivo. Here we took advantage of commercially available high density protein arrays to identify putative target of the two islet specific aptamers. Briefly, 5' biotynilated RNA aptamer 1-717 and m12-3773 were chemically produced , complexed with Alexafluor 647-streptavidin and used as probe on the HuProt™ v2.0 19K protein array. Putative binders were further confirmed by cold target inhibition assays, silencing experiments, and surface plasmon resonance.
Overall design Commercially available human protein arrays were blocked with yeast RNA-enriched blocking buffer and stained with astreptavidin Alexafluor 647 conjugated with 5' biotinylated aptamers. After several washes, images were acquired with a microarray scanner and the fluorescence signal was quantified. Data from 2 different replica (for a total of 4 independent spots per each protein) were background (average fluorescence around the protein spots) corrected, quantile normalized and the differential binding of the aptamer 1-717 and m12-3773 to the spotted protein evaluated.
Contributor(s) Van Simaeys D, De la Fuente A, Zoso A, Zilio S, Grilli A, Williams J, Caroli J, Bicciato S, Serafini P
Citation(s) 35383192
Submission date Nov 27, 2020
Last update date Apr 07, 2022
Contact name Silvio Bicciato
Phone +39-049-827-6108
Organization name University of Padova
Department Molecular Medicine
Street address via U. Bassi 59/b
City Padova
ZIP/Postal code 35131
Country Italy
Platforms (1)
GPL29450 HuProt™ v2.0 19K
Samples (4)
GSM4948215 aptamer 1-717 replicate 1
GSM4948216 aptamer 1-717 replicate 2
GSM4948217 aptamer m12-3773 replicate 1
BioProject PRJNA681228

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Supplementary file Size Download File type/resource
GSE162273_RAW.tar 13.8 Mb (http)(custom) TAR (of GPR)
Processed data included within Sample table

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