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| Status |
Public on Mar 30, 2021 |
| Title |
The chaperonin GroESL supports the functions of the Z-ring regulators FtsA and FzlA |
| Organism |
Caulobacter vibrioides NA1000 |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The highly conserved chaperonin GroESL performs a crucial role in protein folding, however the essential cellular pathways that rely on this chaperone are underexplored. Loss of GroESL leads to severe septation defects in diverse bacteria, suggesting the folding function of GroESL may be integrated with the bacterial cell cycle at the point of cell division. Here, we describe new connections between GroESL and the bacterial cell cycle using the model organism Caulobacter crescentus. Using a proteomics approach, we identify candidate GroESL client proteins that become insoluble or are degraded specifically when GroESL folding is insufficient, revealing several essential proteins that participate in cell division and peptidoglycan biosynthesis. We demonstrate that other cell cycle events such as DNA replication and chromosome segregation are able to continue when GroESL folding is insufficient. We further find that deficiency of two FtsZ-interacting proteins, the bacterial actin homologue FtsA and the constriction regulator FzlA, mediate the GroESL-dependent block in cell division. Our data show that sufficient GroESL is required to maintain normal dynamics of the FtsZ scaffold and divisome functionality in C. crescentus. In addition to supporting divisome function, we show that GroESL is required to maintain the flow of peptidoglycan precursors into the growing cell wall. Linking a chaperone to cell division may be a conserved way to coordinate environmental and internal cues that signal when it is safe to divide.
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| Overall design |
RNA-sequencing was performed to determine gene expression changes induced by depletion of the chaperonin GroES-GroEL (GroESL). RNA abundance in cultures of a GroESL depletion strain (SG300) grown for four hours under depleting conditions was compared to the wild type control.
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| Contributor(s) |
Jonas K, Schroeder K |
| Citation(s) |
33947758 |
| Submission date |
Nov 30, 2020 |
| Last update date |
Jun 29, 2021 |
| Contact name |
Kristina Jonas |
| E-mail(s) |
kristina.jonas@su.se
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| Organization name |
Stockholm University
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| Department |
Department of Molecular Biosciences, The Wenner-Gren Institute
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| Lab |
Jonas lab
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| Street address |
Svante Arrheniusväg 20 C
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| City |
Stockholm |
| ZIP/Postal code |
10691 |
| Country |
Sweden |
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| Platforms (1) |
| GPL26943 |
Illumina HiSeq 2500 (Caulobacter vibrioides NA1000) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA681511 |
| SRA |
SRP295091 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE162320_WT_vs._4hrs_GroESL_depletion.xlsx |
863.7 Kb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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