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Series GSE162413 Query DataSets for GSE162413
Status Public on Nov 01, 2022
Title The cellular basis for epithelial-mesenchymal interaction during mouse tooth development
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary In this study, using mouse molar as the model, we developed a dual fluorescence reporter mouse to precisely track and analyze dental epithelium and mesenchyme at single-cell resolution from early embryonic to postnatal stages. Moreover, we constructed the virtual molar explorer (VMEx) to spatially map 15,967 molar-expressed genes and identified that Msx1+ Sdc1+ marked the developing dental papilla while surrounded by Msx1+ Sdc1- molar niche. Through tooth germ reconstitution and organoid culture in vitro and kidney capsule transplantation in vivo, we provided evidence that the Msx1+ Sdc1- dental follicle cells might function as the tooth organizers that promoted epithelium survival and tooth germ organization. Furthermore, the appearance of Msx1+ Sdc1+ dental papilla cells relied on the interaction between dental epithelium and Msx1+ Sdc1- dental follicle cells. Together, our results revealed the cellular dynamics of tooth development in mice and identified that the dental follicle might be the key driver of epithelial-mesenchymal interaction and tooth morphogenesis.
Overall design Tooth germs were dissected and collected at different development stages, including bud stage (E12.5), cap stage (E14.5), bell stage (E16.5), postnatal day 1 (PN1) and day 7 (PN7). Epithelial and mesenchymal cells were enriched by FACS sorting using copGFP and tdTomato fluorescence. Cells were then used for single-cell RNA sequencing using 10X Genomics platform.
Contributor(s) Duan Y, Fu H, Hu H, Liao Y, Kang F, Zhang Z, Zhang B, Huo F, Yin Y, Chen G, Hu H, Tian W, Li Z
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Submission date Dec 01, 2020
Last update date Nov 03, 2022
Contact name Zhonghan Li
Phone +86-28-85463752
Organization name Sichuan University
Department College of Life Science
Street address 29 Wangjiang Road
City Chengdu
State/province Sichuan
ZIP/Postal code 610064
Country China
Platforms (2)
GPL16417 Illumina MiSeq (Mus musculus)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (5)
GSM4951212 E12.5
GSM4951213 E14.5
GSM4951214 E16.5
BioProject PRJNA681820
SRA SRP295338

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Supplementary file Size Download File type/resource
GSE162413_RAW.tar 1006.2 Mb (http)(custom) TAR (of TAR, ZIP)
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Raw data are available in SRA
Processed data provided as supplementary file

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