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Series GSE162497 Query DataSets for GSE162497
Status Public on Sep 30, 2021
Title Identification of downstream molecules associated with TLR4-dependent inflammatory response in primary bone marrow-derived macrophages (BMDMs) by PolyA-RNA sequencing.
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary To explore the downstream molecules that are responsible for the regulatory role of macrophage-specific TLR4 during inflammatory responses, we analyzed the transcriptome profile of BMDMs isolated from tlr4f/f and tlr4f/f-lysM-Cre mice with or without LPS stimulation by PolyA RNA sequencing.
 
Overall design Primary BMDMs of tlr4f/f and tlr4f/f-lysM-Cre mice were stimulated with 1µg/mL of Lipopolysaccharides (LPS) for 6 hours in vitro. Then, three samples of total mRNAs from each group including control-tlr4f/f, control-tlr4f/f-lysM-cre, LPS-tlr4f/f and LPS-tlr4f/f-lysM-cre were subjected for polyA-RNA sequencing to analyze the effect of macrophage-specific TLR4 on the differential gene expression in response to LPS.
 
Contributor(s) Yang F, Chen J, Huang XR, Yiu WH, Yu X, Tang SC, Lan HY
Citation(s) 34568976
Submission date Dec 02, 2020
Last update date Nov 30, 2021
Contact name Fuye Yang
Organization name Second Affiliated Hospital of Zhejiang University School of Medicine
Department Nephrology
Street address 88 Jiefang Road
City Hanghzou
State/province Zhejiang
ZIP/Postal code 310009
Country China
 
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (12)
GSM4952941 LPS- tlr4f/f BMDM1
GSM4952942 LPS- tlr4f/f BMDM2
GSM4952943 LPS- tlr4f/f BMDM3
Relations
BioProject PRJNA682050
SRA SRP295494

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE162497_Raw_gene_counts_matrix.txt.gz 2.6 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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