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Series GSE162702 Query DataSets for GSE162702
Status Public on Sep 30, 2021
Title RNAseq of Runx1 ck/o B cells at the resting B cell stage and after 3 hours of activation with anti-IgM
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We report the analysis by RNAseq of the changes in gene expression in mature B cells from mice that had the Runx1 gene conditionally knocked out at the transitional B cell stage.
 
Overall design Examination of polyA+ mRNAs from resting B cells isolated from mouse spleen where Runx1 exon 4 was deleted at the transitional B cell stage due to the expression of Cre under the CD23 promoter. The RNAseq analysis was carried out on three replicates for each cell type (control CD23-iCre B cells and CD23-iCre/Runx1 ck/o=Runx1-KO B cells) and for each condition (resting B= 0hrs and 3hrs activation with anti-IgM+IL4). The Runx1 c-k/o / Cd23-Cre conditional knockout mice were generated by crossing the Runx1fl/fl mouse line (generously provided by Nancy Speck, University of Pennsylvania) with the Cd23-Cre transgenic mouse line (generously provided by Meinrad Busslinger, IMP Vienna).
 
Contributor(s) Thomsen I, Dillon N, Sabbattini P, Wang YF
Citation(s) 34810221
Submission date Dec 04, 2020
Last update date Jan 03, 2022
Contact name LMS Bioinformatics Core
E-mail(s) bioinformatics@lms.mrc.ac.uk
Organization name MRC London Institute of Medical Sciences
Department Bioinformatics Core
Street address Hammersmith Hospital Campus, Du Cane Road
City London
ZIP/Postal code W12 0NN
Country United Kingdom
 
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (12)
GSM4957588 CD23-iCre rep1 - restingB (0hrs)
GSM4957589 CD23-iCre rep2 - restingB (0hrs)
GSM4957590 CD23-iCre rep3 - restingB (0hrs)
This SubSeries is part of SuperSeries:
GSE162704 RUNX1 controls the rate of cell cycle entry during naive B cell activation by regulating expression of cell cycle and immunomodulatory genes in response to BCR stimulation
Relations
BioProject PRJNA682638
SRA SRP295993

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Supplementary file Size Download File type/resource
GSE162702_Genecounts.csv.gz 515.3 Kb (ftp)(http) CSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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