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| Status |
Public on Feb 10, 2021 |
| Title |
Single cell RNA-seq of Sox10-Cre::GFP+ population |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We performed single-cell RNA-seq on GFP+ sorted population from the brain from the Sox10-Cre::GFP transgenic mouse at postnatal day P21
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| Overall design |
Brain was dissociated using Neural tissue dissociation kit (P) (Miltenyi Biotec), and debris was removed using Debris removal solution (Miltenyi Biotec). GFP+ cells were sorted on FACS Aria III and single-cell RNA seq was performed using chromium single cell 3' library preparation kit (v3)
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| |
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| Contributor(s) |
Bartosovic M, Kabbe M, Castelo-Branco G |
| Citation(s) |
33846645 |
| |
| Submission date |
Dec 18, 2020 |
| Last update date |
May 13, 2021 |
| Contact name |
Marek Bartosovic |
| E-mail(s) |
bartosovic.marek@gmail.com
|
| Organization name |
Karolinska Institute
|
| Lab |
Goncalo Castelo-Branco
|
| Street address |
Solnavagen 9
|
| City |
Stockholm |
| ZIP/Postal code |
12432 |
| Country |
Sweden |
| |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE163532 |
Single cell profiling of histone modifications and transcription factors in the mouse brain |
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| Relations |
| BioProject |
PRJNA686369 |
| SRA |
SRP298453 |
