|
| Status |
Public on Feb 10, 2021 |
| Title |
Bulk Cut&run and Cut&tag |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We generated bulk Cut&run data with antibodies agains H3K27me3 in primary mouse OPCs and OPC model cell line Oli-neu, and with antibodies agains H3K27ac in Oli-neu cell line with addition of BSA at various steps of the protocol. These datasets were used as benchmarks for the scCUT&Tag data of the mouse brain.
|
| |
|
| Overall design |
Bulk Cut&run and Cut&Tag in primary mouse OPCs and OPC model cell line Oli-neu
|
| |
|
| Contributor(s) |
Bartosovic M, Kabbe M, Castelo-Branco G |
| Citation(s) |
33846645 |
| |
| Submission date |
Dec 18, 2020 |
| Last update date |
May 13, 2021 |
| Contact name |
Marek Bartosovic |
| E-mail(s) |
bartosovic.marek@gmail.com
|
| Organization name |
Karolinska Institute
|
| Lab |
Goncalo Castelo-Branco
|
| Street address |
Solnavagen 9
|
| City |
Stockholm |
| ZIP/Postal code |
12432 |
| Country |
Sweden |
| |
|
| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (6)
|
|
| This SubSeries is part of SuperSeries: |
| GSE163532 |
Single cell profiling of histone modifications and transcription factors in the mouse brain |
|
| Relations |
| BioProject |
PRJNA686390 |
| SRA |
SRP298474 |
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