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Series GSE163637 Query DataSets for GSE163637
Status Public on Dec 06, 2021
Title 10X-single cell seq of B cells during colitis (d0 and d14)
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary B cells expand during the recovery after DSS-induced colonic inflammation. To investigate if a specific subtype is expanding and to analyse the transcriptional profile of these cells a transcriptional analysis on single cell level was carried out. In detail,  B cells were isolated from the colon of mice on day 0 (no treatment) or day 14 (recovery) after 7 days of DSS treatment by flow cytometric sorting and analyzed by 10X sequencing. 
Overall design Female C57BL/6J mice were treated with 2%DSS via the drinking water followed by 7 days of recovery. Lymphocytes were isolated from the colonic lamina propria on day 0 (ctrl) and day 14 (recovery) and B cells were sorted using flow cytometry. Afterwards cells were processed according to 10x Genomics protocol for Chromium Single cell 3’ kit (version 2) and submitted for sequencing
Contributor(s) Frede A, Sorini C, Tripathi KP
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Submission date Dec 21, 2020
Last update date Dec 07, 2021
Contact name Eduardo J Villablanca
Organization name Karolinska Institute
Department CMM
Street address visiongatan 18
City Stockholm
ZIP/Postal code 17176
Country Sweden
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (2)
GSM4983263 B0: B cell population at day 0 scRNA-seq
GSM4983264 B14: B cell population at day 14 scRNA-seq
BioProject PRJNA686980
SRA SRP298743

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Supplementary file Size Download File type/resource
GSE163637_RAW.tar 89.2 Mb (http)(custom) TAR (of MTX, TSV, TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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