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| Status |
Public on Dec 06, 2021 |
| Title |
10X-single cell seq of B cells during colitis (d0 and d14) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
B cells expand during the recovery after DSS-induced colonic inflammation. To investigate if a specific subtype is expanding and to analyse the transcriptional profile of these cells a transcriptional analysis on single cell level was carried out. In detail, B cells were isolated from the colon of mice on day 0 (no treatment) or day 14 (recovery) after 7 days of DSS treatment by flow cytometric sorting and analyzed by 10X sequencing.
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| Overall design |
Female C57BL/6J mice were treated with 2%DSS via the drinking water followed by 7 days of recovery. Lymphocytes were isolated from the colonic lamina propria on day 0 (ctrl) and day 14 (recovery) and B cells were sorted using flow cytometry. Afterwards cells were processed according to 10x Genomics protocol for Chromium Single cell 3’ kit (version 2) and submitted for sequencing
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| Contributor(s) |
Frede A, Sorini C, Tripathi KP |
| Citation missing |
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| |
| Submission date |
Dec 21, 2020 |
| Last update date |
Dec 07, 2021 |
| Contact name |
Eduardo J Villablanca |
| E-mail(s) |
eduardo.villablanca@ki.se
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| Organization name |
Karolinska Institute
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| Department |
CMM
|
| Street address |
visiongatan 18
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| City |
Stockholm |
| ZIP/Postal code |
17176 |
| Country |
Sweden |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (2) |
| GSM4983263 |
B0: B cell population at day 0 scRNA-seq |
| GSM4983264 |
B14: B cell population at day 14 scRNA-seq |
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| Relations |
| BioProject |
PRJNA686980 |
| SRA |
SRP298743 |
