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Series GSE163707 Query DataSets for GSE163707
Status Public on Mar 01, 2021
Title Human plasmacytoid dendritic cells mount a distinct antiviral response to virus-infected cells
Organisms Homo sapiens; blank sample
Experiment type Expression profiling by high throughput sequencing
Summary Plasmacytoid dendritic cells (pDCs) can rapidly produce interferons and other soluble factors in response to extracellular viruses or virus mimics such as CpG-containing DNA. pDCs can also recognize live cells infected with certain RNA viruses, but the relevance and functional consequences of such recognition remain unclear. We studied the response of primary DCs to the prototypical persistent DNA virus, the human cytomegalovirus (CMV). Human pDCs responded poorly to free CMV but strongly to live CMV-infected fibroblasts, in a process that involved integrin-mediated adhesion, transfer of viral DNA to pDCs and its recognition through TLR9. Compared to transient polyfunctional responses to CpG or free influenza virus, pDC response to CMV-infected cells was long-lasting, dominated by the production of type I (IFN-I) and type III (IFN-III) interferons, and lacked diversification into functionally distinct populations. Similarly, pDC activation by influenza-infected lung epithelial cells was highly efficient, prolonged and dominated by interferon production. Prolonged pDC activation by CMV-infected cells facilitated the activation of natural killer cells that are critical for CMV control. Finally, patients with CMV viremia harbored phenotypically activated pDCs and increased levels of IFN-I and IFN-III in circulation. Thus, recognition of live infected cells is a common mechanism of virus detection by pDCs that elicits a unique antiviral response program.
Overall design RNA-sequencing of pDCs treated with CpG, CMV, and both infected and uninfected MRC5 cells using PLATE-seq 3'-tag sequencing
Contributor(s) Yun TJ, Sims PA, Reizis B
Citation(s) 33811059
Submission date Dec 22, 2020
Last update date May 31, 2021
Contact name Peter A Sims
Organization name Columbia University
Street address 3960 Broadway, Lasker 203AC
City New York
State/province NY
ZIP/Postal code 10032
Country USA
Platforms (2)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
GPL29107 Illumina NextSeq 500 (blank sample)
Samples (96)
GSM4984119 BR.a1
GSM4984120 BR.a2
GSM4984121 BR.a3
BioProject PRJNA687242
SRA SRP298947

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Supplementary file Size Download File type/resource
GSE163707_BR.matrix.txt.gz 1.9 Mb (ftp)(http) TXT
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