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Series GSE163742 Query DataSets for GSE163742
Status Public on May 04, 2021
Title RNA-Seq of Col-0, dek3-2, dek4-2, and dek3-2 dek4-2 (dd-2) 7-day-old seedlings in Arabidopsis
Organism Arabidopsis thaliana
Experiment type Expression profiling by high throughput sequencing
Summary Evolutionarily conserved DEK domain-containing proteins have been implicated in multiple chromatin-related processes, mRNA splicing, and transcriptional regulation in eukaryotes.
Here, we show that two DEK proteins, DEK3 and DEK4, redundantly control the floral transition in Arabidopsis. DEK3 and DEK4 directly associate with chromatin of related flowering repressors, FLOWERING LOCUS C (FLC), and its two homologs, MADS AFFECTING FLOWERING4 (MAF4) and MAF5, to promote their expression.
The binding of DEK3 and DEK4 to histone octamer in vivo affects histone modifications at FLC, MAF4 and MAF5 loci. In addition, DEK3 and DEK4 interact with RNA polymerase II and promote the association of RNA polymerase II with FLC, MAF4 and MAF5 chromatin to promote their expression.
Our results show that DEK3 and DEK4 directly interact with chromatin to facilitate the transcription of key flowering repressors and thus prevent precocious flowering in Arabidopsis.
Overall design 7-day-old seedlings of Col-0, dek3-2, dek4-2, and dd-2 were examined with RNA-Seq to identify differentially expressed genes caused by DEK3 and DEK4 mutation in Arabidopsis.
8 samples from Col-0, dek3-2, dek4-2, and dd-2 7-day-old seedlings grown under long day condition with two biological replicates.
Contributor(s) Zong W, Sung S
Citation(s) 33774831
Submission date Dec 23, 2020
Last update date May 04, 2021
Contact name Wei Zong
Organization name University of Texas at Austin
Department Molecular Biosciences
Street address NMS 5.268, Stop A5000
City Austin
State/province Texas
ZIP/Postal code 78712
Country USA
Platforms (1)
GPL19580 Illumina NextSeq 500 (Arabidopsis thaliana)
Samples (8)
GSM4985008 Col-0_r1
GSM4985009 Col-0_r2
GSM4985010 dek3-2_r1
BioProject PRJNA687415
SRA SRP299050

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE163742_RAW.tar 7.1 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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