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Series GSE163937 Query DataSets for GSE163937
Status Public on Mar 10, 2021
Title Transcriptome analysis of mercury chloride (HgCl2) treated H1299 human non-small cell lung carcinoma cells
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Purpose: finding differential transcripts usage in environmentally harzardous chemical, mercury chloride (HgCl2) in human non-small cell lung carcinoma cells
Methods: transcriptome analysis by the RNA-seq via mRNA pull-down
Results: We found that HgCl2 increases cellular toxicity by the increasing of apoptosis via caspase-3 independent pathway in H12999 cells. Also, over thounds of altered gene expression pattern were detected from the RNA-seq analysis.
Conclusions: mercury chloride (HgCl2) increases cytotoxic effects and apoptosis in H1299 cells through the caspase-3 independent pathway and changes the context of trascript
 
Overall design H1299 cells were treated with various concentration of mercury chloride and all experiments were duplicated.
 
Contributor(s) Kim J
Citation(s) 33670495
Submission date Dec 28, 2020
Last update date Mar 10, 2021
Contact name Jung-Woong Kim
E-mail(s) jungkim@cau.ac.kr
Organization name Chung-Ang University
Department Life Science
Lab Genome Dynamics Lab
Street address 84 Heuksuk-ro Dongjak-ku
City Seoul
ZIP/Postal code 06974
Country South Korea
 
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (4)
GSM4990856 EtOH rep1
GSM4990857 EtOH rep2
GSM4990858 HgCl2 rep1
Relations
BioProject PRJNA688255
SRA SRP299484

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE163937_normalized_counts_FPKM_.txt.gz 1.7 Mb (ftp)(http) TXT
GSE163937_raw_counts.txt.gz 801.2 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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