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Series GSE164098 Query DataSets for GSE164098
Status Public on Mar 23, 2021
Title Suppression of the NTS-CPS1 regulatory axis by AFF1 in lung adenocarcinoma cells
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Expression profiling by high throughput sequencing
Summary Up-regulation of the neuropeptide NTS in a subgroup of lung cancers has been linked to poor prognosis. However, the regulatory pathway centered on NTS in lung cancer remains unclear. Here we identified the NTS specific enhancer in lung adenocarcinoma cells. The AF4/FMR2 (AFF) family protein AFF1 occupies the NTS enhancer and inhibits NTS transcription. Clustering analysis of lung adenocarcinoma gene expression data demonstrated that NTS is highly positively correlated with the expression of the oncogenic factor CPS1. Detailed analyses demonstrated that NTS antagonizes the IL6 pathway in regulating CPS1. Thus, our analyses revealed a novel NTS centered regulatory axis, consisting of AFF1 as a master transcription suppressor and IL6 as an antagonist in lung adenocarcinoma cells.
Overall design AFF1 ChIP sample.
RNA-seq in control and AFF1 knockdown A549 cells were performed in duplicates.
Contributor(s) Liu X, Lin C
Citation(s) 33493519
Submission date Dec 31, 2020
Last update date Mar 23, 2021
Contact name Xiaoxu Liu
Organization name Southeast University
Department Institute of Life Sciences
Lab the Key Laboratory of Developmental Genes and Human Disease
Street address 2 Sipailou
City Nanjing
State/province Jiangsu
ZIP/Postal code 210018
Country China
Platforms (1)
GPL9052 Illumina Genome Analyzer (Homo sapiens)
Samples (5)
GSM4996646 AFF1
GSM5023328 SHC_1
GSM5023329 SHC_2
BioProject PRJNA688896
SRA SRP299901

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Supplementary file Size Download File type/resource
GSE164098_RAW.tar 280.3 Mb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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