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Series GSE164270 Query DataSets for GSE164270
Status Public on Jun 08, 2021
Title Reovirus low-density particles package viral and cellular RNA
Organisms Mus musculus; Mammalian orthoreovirus 1 Lang; Mammalian orthoreovirus 3 Dearing; blank sample
Experiment type Expression profiling by high throughput sequencing
Summary Packaging of segmented, double-stranded RNA viral genomes requires coordination of multiple viral proteins and RNA segments. For mammalian orthoreovirus (reovirus), evidence suggests either all ten or zero viral RNA segments are simultaneously packaged in a highly coordinated process hypothesized to exclude host RNA. Accordingly, reovirus generates genome-containing virions and “genomeless” top component particles. However, despite ostensibly lacking the genome, top component particles maintain a low level of infectivity. Whether reovirus particles can package host RNA is unknown. To gain insight into reovirus packaging potential and mechanisms, we employed next-generation RNA-sequencing to define the viral and host RNA content of purified reovirus virions and top component particles. Reovirus top component particles contained double-stranded viral RNA segments in similar proportions but at reduced levels compared to virions. Top component particles also were enriched for numerous host RNAs, especially short, non-polyadenylated transcripts, that differed by reovirus strain, independent of the viral polymerase. In contrast, virions were enriched for very few host RNAs. Collectively, these findings indicate that genome packaging into reovirus virions is exquisitely selective, while incorporation of host RNAs into top component particles is more promiscuous or differentially selective and may contribute to or result from inefficient viral RNA packaging.
 
Overall design Examination of RNA component in the Top component of reovirus
 
Contributor(s) Thoner TW Jr, Ogden KM
Citation(s) 34201386
Submission date Jan 05, 2021
Last update date Jul 15, 2021
Contact name John Karijolich
E-mail(s) john.karijolich@vumc.org
Organization name Vanderbilt University Medical Center
Department PMI
Lab John Karijolich Lab
Street address 1211 Medical Center Drive
City Nashville
State/province TN
ZIP/Postal code 37232
Country USA
 
Platforms (6)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
GPL29487 Illumina NovaSeq 6000 (blank sample)
GPL29563 Illumina NovaSeq 6000 (Mammalian orthoreovirus 1 Lang)
Samples (18)
GSM5005568 T1L reovirus replicate 1
GSM5005569 T1L reovirus replicate 2
GSM5005570 T3D reovirus replicate 1
Relations
BioProject PRJNA689770
SRA SRP300447

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Supplementary file Size Download File type/resource
GSE164270_raw_counts.csv.gz 938.4 Kb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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