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Series GSE164709 Query DataSets for GSE164709
Status Public on Mar 18, 2021
Title Apoptotic cells induce proliferation of peritoneal macrophages
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The interaction of macrophages with apoptotic cells is required for efficient resolution of inflammation. While apoptotic cell removal prevents inflammation due to secondary necrosis, it also alters the macrophage phenotype to hinder further inflammatory reactions. The interaction between apoptotic cells and macrophages is often studied by chemical or biological induction of apoptosis, which may introduce artifacts by affecting the macrophages as well and/or triggering unrelated signaling pathways. Here, we set up a pure cell death system in which NIH 3T3 cells expressing dimerizable Caspase-8 were co-cultured with peritoneal macrophages in a transwell system. Phenotype changes in macrophages induced by apoptotic cells were evaluated by RNA sequencing, which revealed an unexpectedly dominant impact on macrophage proliferation. This was confirmed in functional assays with primary peritoneal macrophages and IC-21 macrophages. Moreover, inhibition of apoptosis during Zymosan-induced peritonitis in mice decreased mRNA levels of cell cycle mediators in peritoneal macrophages. Proliferation of macrophages in response to apoptotic cells may be important to increase macrophage numbers to allow efficient clearance and resolution of inflammation.
 
Overall design mRNA profiles of peritoneal macrophages after co-culture with apoptotic cells
 
Contributor(s) Knuth A, Rappl P, Bauer R, Mota AC, Schmid T, Fulda S, Weigert A
Citation(s) 33668084
Submission date Jan 12, 2021
Last update date Mar 18, 2021
Contact name Anne-Kathrin Knuth
E-mail(s) a.knuth@kinderkrebsstiftung-frankfurt.de
Organization name Institut of experimental cancer research in pediatrics
Lab Fulda
Street address Komturstra├če 3a
City Frankfurt am Main
ZIP/Postal code 60528
Country Germany
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (8)
GSM5017653 Macrophages_rep1
GSM5017654 Macrophages_rep2
GSM5017655 Macrophages_rep3
Relations
BioProject PRJNA691612
SRA SRP301469

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE164709_MacroApopt_normalized_counts.xlsx 1.8 Mb (ftp)(http) XLSX
GSE164709_MacroCtrl_normalized_counts.xlsx 1.8 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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