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| Status |
Public on Mar 18, 2021 |
| Title |
Apoptotic cells induce proliferation of peritoneal macrophages |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The interaction of macrophages with apoptotic cells is required for efficient resolution of inflammation. While apoptotic cell removal prevents inflammation due to secondary necrosis, it also alters the macrophage phenotype to hinder further inflammatory reactions. The interaction between apoptotic cells and macrophages is often studied by chemical or biological induction of apoptosis, which may introduce artifacts by affecting the macrophages as well and/or triggering unrelated signaling pathways. Here, we set up a pure cell death system in which NIH 3T3 cells expressing dimerizable Caspase-8 were co-cultured with peritoneal macrophages in a transwell system. Phenotype changes in macrophages induced by apoptotic cells were evaluated by RNA sequencing, which revealed an unexpectedly dominant impact on macrophage proliferation. This was confirmed in functional assays with primary peritoneal macrophages and IC-21 macrophages. Moreover, inhibition of apoptosis during Zymosan-induced peritonitis in mice decreased mRNA levels of cell cycle mediators in peritoneal macrophages. Proliferation of macrophages in response to apoptotic cells may be important to increase macrophage numbers to allow efficient clearance and resolution of inflammation.
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| |
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| Overall design |
mRNA profiles of peritoneal macrophages after co-culture with apoptotic cells
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| |
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| Contributor(s) |
Knuth A, Rappl P, Bauer R, Mota AC, Schmid T, Fulda S, Weigert A |
| Citation(s) |
33668084 |
| |
| Submission date |
Jan 12, 2021 |
| Last update date |
Mar 18, 2021 |
| Contact name |
Anne-Kathrin Knuth |
| E-mail(s) |
a.knuth@kinderkrebsstiftung-frankfurt.de
|
| Organization name |
Institut of experimental cancer research in pediatrics
|
| Lab |
Fulda
|
| Street address |
Komturstraße 3a
|
| City |
Frankfurt am Main |
| ZIP/Postal code |
60528 |
| Country |
Germany |
| |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA691612 |
| SRA |
SRP301469 |
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