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Series GSE16497 Query DataSets for GSE16497
Status Public on Jun 10, 2009
Title Arabidopsis thaliana gene expression changes upon treatment with green peach aphid saliva
Organism Arabidopsis thaliana
Experiment type Expression profiling by array
Summary Myzus persicae (green peach aphid) feeding on Arabidopsis thaliana induces a defense response, quantified as reduced aphid progeny production, in infested leaves but not in other parts of the plant. Similarly, infiltration of aphid saliva into Arabidopsis leaves causes only a local increase in aphid resistance. Further characterization of the defense-eliciting salivary components indicates that Arabidopsis recognizes a proteinaceous elicitor with a size between 3 to 10 kD. Genetic analysis using well-characterized Arabidopsis mutant shows that saliva-induced resistance against M. persicae is independent of the known defense signaling pathways involving salicylic acid, jasmonate, and ethylene. Among 78 Arabidopsis genes that were induced by aphid saliva infiltration, 52 had been identified previously as aphid-induced, but few are responsive to the well-known plant defense signaling molecules salicylic acid and jasmonate. Quantitative PCR analysis confirms expression of saliva-induced genes. In particular, expression of a set of O-methyltransferases, which may be involved in the synthesis of aphid-repellent glucosinolates, was significantly up-regulated by both M. persicae feeding and treatment with aphid saliva. However, this did not correlate with increased production of 4-methoxyindol-3-ylmethylglucosinolate, suggesting that aphid salivary components trigger an Arabidopsis defense response that is independent of this aphid-deterrent glucosinolate.
Overall design 3 biological replicates (control and treatment). Total number of samples: 6.
Contributor(s) Jander G, de Vos M
Citation(s) 19558622
Submission date Jun 08, 2009
Last update date Aug 28, 2018
Contact name Georg Jander
Phone +1 607 254 1365
Organization name Boyce Thompson Institute for Plant Research
Street address 1 Tower Rd
City Ithaca
State/province NY
ZIP/Postal code 14853
Country USA
Platforms (1)
GPL198 [ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array
Samples (6)
GSM414632 control, rep 1
GSM414633 treatment, rep 1
GSM414634 control, rep 2
BioProject PRJNA116115

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE16497_Jander_NormalizedFoldChange.txt.gz 520.1 Kb (ftp)(http) TXT
GSE16497_RAW.tar 13.0 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
Processed data are available on Series record

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