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Series GSE165150 Query DataSets for GSE165150
Status Public on Mar 18, 2021
Title Gene expression analysis of single and co-cultured HER2 L755S mutants in MCF-10A background
Organism Homo sapiens
Experiment type Expression profiling by RT-PCR
Summary PIK3CA E545K mutants induce epidermal growth-factor independent proliferation of HER2 L755S mutants through a contact-mediated mechanism.
We used RT2 Profiler PCR Arrays to study changes in gene expression in the HER2 L755S mutants when co-cultured with the PIK3CA E545K mutants and compared it to the HER2 L755S mutants grown in isolation.
 
Overall design There are two groups. HER2 L755S mutants were co-cultured with PIK3CA E545K mutants. The co-culture was then flow-sorted to separate the HER2 L755S mutants. The flow-sorted HER2 L755S mutants were the test group. HER2 L755S mutants grown in isolation were used as the control group.
Each group (test and control) had two biological replicates and each biological replicate had two technical replicates (quadrapilcates for each group in total).
 
Contributor(s) Karthikeyan S
Citation(s) 33529175
Submission date Jan 19, 2021
Last update date Mar 18, 2021
Contact name Swathi Karthikeyan
E-mail(s) swathikarthikeyn@gmail.com
Organization name Johns Hopkins University School of Medicine
Street address 733 N Broadway
City Baltimore
State/province MD
ZIP/Postal code 21205
Country USA
 
Platforms (1)
GPL29628 Qiagen RT2 Profiler PCR Array Human EGF/PDGF Signaling Pathway [PAHS-040Z]
Samples (2)
GSM5027096 Co-cultured and flow-sorted HER2 L755S mutants
GSM5027097 HER2 L755S mutants grown in isolation
Relations
BioProject PRJNA693352

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE165150_Fold_change_worksheet_Karthikeyan_et_al..xlsx 14.2 Kb (ftp)(http) XLSX
GSE165150_Matrix_non-normalized_worksheet_Karthikeyan_et_al..xlsx 15.2 Kb (ftp)(http) XLSX
Processed data included within Sample table
Processed data are available on Series record

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