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Status |
Public on May 11, 2021 |
Title |
The effect of diet on H3K9-butyryl and H3K9-acetyl in the heart before and after transverse aortic constriction in C57Bl/6J mice |
Organism |
Mus musculus |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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Summary |
Mice (C57Bl/6J) that are fed increasing levels of dietary fat (0, 10, and 60 Kcal%) show a parallel increasing decline in global promoter-H3K9-butyryl (H3K9Bu), but none in promoter-H3K9-acetyl (H3K9ac), in the heart. Additionally, transverse aortic constriction (TAC) induces a further decline in H3K9Bu, compared to an increase in H3K9ac.
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Overall design |
C57Bl/6J mice were fed either a fat-free (0 Kcal%, Fat 0), low-fat (10 Kcal%, Fat 10 or control), or high-fat (60 Kcal%, Fat 60 or HiFat) diet for 4 days before subjecting them to a sham or transverse aortic constriction (TAC) surgeries. The mice were maintained on the same diet for another week, before they were assessed by echocardiography, the hearts isolated, chromatin extracted , and subjected to anti-H3K9Bu and anti-H3K9ac ChIP-Seq
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Contributor(s) |
Adellatif M |
Citation(s) |
33989779 |
NIH grant(s) |
Grant ID |
Grant title |
Affiliation |
Name |
R01 HL146537 |
Transcriptional mechanisms in cardiac hypertrophy |
RUTGERS, THE STATE UNIVERSITY OF NEW JERSEY--RBHS-NEW JERSEY MEDICAL SCHOOL |
Maha Abdellatif |
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Submission date |
Jan 21, 2021 |
Last update date |
Jun 28, 2021 |
Contact name |
Maha Abdellatif |
E-mail(s) |
abdellma@njms.rutgers.edu
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Phone |
9739721254
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Organization name |
Rutgers-University
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Department |
Cell Biology and Molecular Medicine
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Street address |
185 S. Orange Ave.
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City |
Newark |
State/province |
NJ |
ZIP/Postal code |
07103 |
Country |
USA |
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Platforms (1) |
GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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Samples (14)
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Relations |
BioProject |
PRJNA693860 |
SRA |
SRP302756 |