GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE165279 Query DataSets for GSE165279
Status Public on May 11, 2021
Title The effect of diet on H3K9-butyryl and H3K9-acetyl in the heart before and after transverse aortic constriction in C57Bl/6J mice
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Mice (C57Bl/6J) that are fed increasing levels of dietary fat (0, 10, and 60 Kcal%) show a parallel increasing decline in global promoter-H3K9-butyryl (H3K9Bu), but none in promoter-H3K9-acetyl (H3K9ac), in the heart. Additionally, transverse aortic constriction (TAC) induces a further decline in H3K9Bu, compared to an increase in H3K9ac.
Overall design C57Bl/6J mice were fed either a fat-free (0 Kcal%, Fat 0), low-fat (10 Kcal%, Fat 10 or control), or high-fat (60 Kcal%, Fat 60 or HiFat) diet for 4 days before subjecting them to a sham or transverse aortic constriction (TAC) surgeries. The mice were maintained on the same diet for another week, before they were assessed by echocardiography, the hearts isolated, chromatin extracted , and subjected to anti-H3K9Bu and anti-H3K9ac ChIP-Seq
Contributor(s) Adellatif M
Citation(s) 33989779
NIH grant(s)
Grant ID Grant title Affiliation Name
R01 HL146537 Transcriptional mechanisms in cardiac hypertrophy RUTGERS, THE STATE UNIVERSITY OF NEW JERSEY--RBHS-NEW JERSEY MEDICAL SCHOOL Maha Abdellatif
Submission date Jan 21, 2021
Last update date Jun 28, 2021
Contact name Maha Abdellatif
Phone 9739721254
Organization name Rutgers-University
Department Cell Biology and Molecular Medicine
Street address 185 S. Orange Ave.
City Newark
State/province NJ
ZIP/Postal code 07103
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (14)
GSM5029366 Sham-Fat 0-H3K9ac
GSM5029367 TAC-Fat 0-H3K9ac
GSM5029368 Sham-Fat 0-H3K9Bu
BioProject PRJNA693860
SRA SRP302756

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE165279_RAW.tar 1.8 Gb (http)(custom) TAR (of BW, XLSX)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap