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| Status |
Public on Jun 30, 2023 |
| Title |
single cell RNA Sequencing of hippocampi of mice subjected or not to the learned helplessness paradigm |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The goal of the study was to identify the hippocampal microenvironment asscoaited with stress, in particular stress-induced learned helplessness. By comparing chronic and acute induction of learned helplessness, we identified cell populations and genes associated with resilience.
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| Overall design |
Mice were subjected or not to the learned helplessness paradigms (either acute: mice received footshocks and were tested the next day, or chronic: mice received the acute paradigm and are retested for 3 weeks, weekly), and hippocampal CD45+ live dead - cells were sorted, and 10X Genomics single cell RNA Sequencing was performed.
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| Contributor(s) |
Beurel E |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 MH110415 |
The microbiota, a possible link between Th17 cells and depression |
University of Miami School of Medicine |
Eleonore Beurel |
| R01 MH104656 |
Th17 cells as a new therapeutic target for depression |
University of Miami School of Medicine |
Eleonore Beurel |
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| |
| Submission date |
Jan 25, 2021 |
| Last update date |
Jun 30, 2023 |
| Contact name |
Eleonore Beurel |
| E-mail(s) |
ebeurel@miami.edu
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| Phone |
2059832516
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| Organization name |
University of Miami, Miller School of Medicine
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| Department |
Psychiatry
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| Street address |
1011 NW 15th Street Gautier room 415
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| City |
Miami |
| State/province |
FL |
| ZIP/Postal code |
33136 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (6)
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| GSM5034812 |
ND4w (nonlearned helpless in the chronic paradigm) |
| GSM5034813 |
NR4w (non recovered) |
| GSM5034814 |
R4w (recovered, mice were learned helpless and stop being learned helpless overtime) |
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| Relations |
| BioProject |
PRJNA694767 |
| SRA |
SRP303241 |
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