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Series GSE165732 Query DataSets for GSE165732
Status Public on Apr 14, 2021
Title Ribosome quality control activity potentiates vaccinia virus protein synthesis during infection
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Viral infection both activates stress signaling pathways and redistributes ribosomes away from host mRNAs to translate viral mRNAs. The intricacies of this ribosome shuffle from host to viral mRNAs are poorly understood
Here, we uncover a role for the ribosome associated quality control (RQC) factor, ZNF598, during vaccinia virus mRNA translation. ZNF598 acts on collided ribosomes to ubiquitylate 40S subunit proteins uS10 and eS10 initiating RQC-dependent nascent chain degradation and ribosome recycling
We show that vaccinia infection in human cells enhances uS10 ubiquitylation indicating an increased burden on RQC pathways during viral propagation. Consistent with an increased RQC demand, we demonstrate that vaccinia virus replication is impaired in cells which either lack ZNF598 or express a ubiquitylation deficient version of uS10
Using SILAC-based proteomics and concurrent RNAseq analysis, we determine that translation and not transcription of vaccinia virus mRNAs is compromised in cells with deficient RQC activity. Additionally, vaccinia virus infection reduces cellular RQC activity, suggesting that co-option of ZNF598 by vaccinia virus plays a critical role in translational reprogramming that is needed for optimal viral propagation.
Overall design 6 samples were analyzed in triplicates
Contributor(s) Sundaramoorthy E, Bennett E
Citation(s) 33912921
Submission date Jan 28, 2021
Last update date May 20, 2021
Contact name Elayanambi Sundaramoorthy
Organization name University of California San Diego
Department Section of cell and developmental biology
Lab Bennett lab
Street address 9500, GILMAN DRIVE, la jolla
City san diego
State/province California
ZIP/Postal code 92037
Country USA
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (18)
GSM5048996 HCT-116- mock -replicate 1 [PAR_1_BC]
GSM5048997 HCT-116- mock -replicate 2 [PAR_2_BC]
GSM5048998 HCT-116- mock -replicate 3 [PAR_3_BC]
BioProject PRJNA695555
SRA SRP303696

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Supplementary file Size Download File type/resource
GSE165732_MATRIX_TABLE_rqc_final.txt.gz 679.7 Kb (ftp)(http) TXT
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