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| Status |
Public on Jan 30, 2021 |
| Title |
miRNA expression in tracheal aspirates in mild/moderate and severe BPD |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by RT-PCR
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| Summary |
Extreme preterm infants are a growing population in neonatal intensive care units who carry a high mortality and morbidity. Multiple factors play a role in preterm birth, resulting in major impact on organogenesis leading to complications including bronchopulmonary dysplasia (BPD). The goal of this study was to identify biomarker signatures associated with BPD severity. We analyzed profiles in tracheal aspirates (TAs) from 25 extremely preterm infants receiving invasive mechanical ventilation. Eight infants were diagnosed with mild/moderate BPD, and 17 were diagnosed with severe BPD, according to the NHLBI consensus conference classification . We found specific miRNA signatures in TAs that may serve as biomarkers for BPD severity.
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| Overall design |
We extracted RNA from 0.5 mL of tracheal aspirates from premature neonates with BPD using the Plasma/Serum RNA Purification Kit. The expression of 1,066 human miRNAs was quantified using the Human miRNome miScript® miRNA PCR Array (QIAGEN, MIHS-3216Z). Results (Ct values) were normalized to global means, and fold changes were calculated using the 2-ΔΔCT method.
mild/moderate BPD: 5,17,25,68,79,82,85,97 severe BPD: 3,11,12,24,26,33,34,37,42,49,51,67,74,80,91,95,100
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| Contributor(s) |
Silveyra P, Siddaiah R, Oji-Mmuo C, Fuentes N, Montes D |
| Citation(s) |
33807742 |
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| Submission date |
Jan 29, 2021 |
| Last update date |
May 01, 2021 |
| Contact name |
Patricia Silveyra |
| E-mail(s) |
psilveyr@iu.edu, ptrcslvr@gmail.com
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| Organization name |
Indiana University Bloomington
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| Department |
Environmental and Occupational Health
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| Lab |
Silveyra lab
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| Street address |
2819 E 10th St
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| City |
Bloomington |
| State/province |
Indiana |
| ZIP/Postal code |
47408 |
| Country |
USA |
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| Platforms (1) |
| GPL26552 |
Qiagen Human miRNome miScript miRNA PCR Array (MIHS-3216Z) |
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| Samples (25)
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| Relations |
| BioProject |
PRJNA697980 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE165828_RAW.tar |
30.0 Kb |
(http)(custom) |
TAR |
| GSE165828_fold-change_data.txt.gz |
113.2 Kb |
(ftp)(http) |
TXT |
| GSE165828_non-normalized_data.txt.gz |
119.1 Kb |
(ftp)(http) |
TXT |
| GSE165828_normalized_data.txt.gz |
125.6 Kb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
| Processed data are available on Series record |
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