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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jul 22, 2009 |
| Title |
miRNA expression in cytokine-stimulated airway smooth muscle cells |
| Platform organisms |
Homo sapiens; Mus musculus; Rattus norvegicus |
| Sample organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
We used microarrays to determine if miRNA expression in human airway smooth muscle cells is altered by a pro-inflammatory stimulus. A majority of the miRNAs on the array exhibited very low signal intensity. In ASM cells exposed to IL-1β, TNFα, and IFNγ, none of the miRNA expressed were significantly up-regulated with cytokine treatment. We did observe 11 miRNA down-regulated > 2-fold in both cultures with cytokine treatment. These miRNA include described human miRNA miR-23a, -23b, -25, -188, -320, -363, -489, as well as mouse and rat miRNA homologous to miR-140*, mouse miR-329 and a novel miRNA, abi-13268.
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| Overall design |
miRNA arrays for cytokine-stimulated ASM cells were completed in duplicate from two different cultures to identify candidate miRNA for further study. Cultures were growth arrested for 48 hours and treated with 10 ng/ml IL-1β, TNFα, and IFNγ for 24 hrs. A comparison of miRNA expression under cytokine-stimulated and non-treated conditions from hybridized miRNA arrays was calculated as described.
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| Contributor(s) |
Kuhn AR, Schlauch K, Halayko AJ, Gerthoffer WT, Singer CA |
| Citation(s) |
19541842 |
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| Submission date |
Jun 12, 2009 |
| Last update date |
Mar 21, 2012 |
| Contact name |
Cherie A Singer |
| E-mail(s) |
csinger@medicine.nevada.edu
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| Organization name |
University of Nevada School of Medicine
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| Department |
Pharmacology MS318
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| Street address |
1664 N. Virginia St
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| City |
Reno |
| State/province |
NV |
| ZIP/Postal code |
89557 |
| Country |
USA |
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| Platforms (1) |
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| Samples (4)
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| GSM416913 |
miRNA expression in cytokine-stimulated airway smooth muscle cells, replicate 1.1 |
| GSM416918 |
miRNA expression in cytokine-stimulated airway smooth muscle cells, replicate 1.2 |
| GSM416919 |
miRNA expression in cytokine-stimulated airway smooth muscle cells, replicate 2.1 |
| GSM416921 |
miRNA expression in cytokine-stimulated airway smooth muscle cells, replicate 2.2 |
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| Relations |
| BioProject |
PRJNA116149 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE16587_RAW.tar |
850.0 Kb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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