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| Status |
Public on Feb 04, 2021 |
| Title |
Microarray comparative analysis of wild-type and hd1-3 mutant rice |
| Organism |
Oryza sativa |
| Experiment type |
Expression profiling by array
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| Summary |
Heading date1(Hd1) is a critical regulator controlling rice flowering time, which promotes flowering under short-day (SD) conditions and represses flowering under long-day (LD) conditions. In our previous study (Luan et al., 2009), we identified a rice mutant, hd1-3, in which the Hd1 gene was deficient due to several insertions/deletions in the coding region. To search for downstream genes regulated by Hd1, we performed microarray analysis of hd1-3 mutant and the wild-type Zhonghua11 under both SD and LD conditions. According to the microarray results, SDG712 gene was significantly downregulated in the hd1-3 mutant, indicating that SDG712 gene may acts downstream of Hd1, and may functions in rice flowering time regulation.
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| Overall design |
The leaves of 35-day-old plants of hd1-3 mutant and the wild-type Zhonghua11 grown under SD and LD conditions were collected. Total RNAs were extracted using TRIzol and mRNAs were purified for microarray analysis.
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| Contributor(s) |
Zhang S, Ma X, Luan W |
| Citation(s) |
34357443 |
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| Submission date |
Feb 03, 2021 |
| Last update date |
Aug 24, 2021 |
| Contact name |
Xuan Ma |
| E-mail(s) |
skyxma@tjnu.edu.cn
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| Organization name |
Tianjin Normal University
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| Department |
College of Life Sciences
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| Street address |
Binshui West Road
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| City |
Tianjin |
| ZIP/Postal code |
300387 |
| Country |
China |
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| Platforms (1) |
| GPL2025 |
[Rice] Affymetrix Rice Genome Array |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA699148 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE166053_RAW.tar |
20.3 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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