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Series GSE166053 Query DataSets for GSE166053
Status Public on Feb 04, 2021
Title Microarray comparative analysis of wild-type and hd1-3 mutant rice
Organism Oryza sativa
Experiment type Expression profiling by array
Summary Heading date1(Hd1) is a critical regulator controlling rice flowering time, which promotes flowering under short-day (SD) conditions and represses flowering under long-day (LD) conditions. In our previous study (Luan et al., 2009), we identified a rice mutant, hd1-3, in which the Hd1 gene was deficient due to several insertions/deletions in the coding region. To search for downstream genes regulated by Hd1, we performed microarray analysis of hd1-3 mutant and the wild-type Zhonghua11 under both SD and LD conditions. According to the microarray results, SDG712 gene was significantly downregulated in the hd1-3 mutant, indicating that SDG712 gene may acts downstream of Hd1, and may functions in rice flowering time regulation.
Overall design The leaves of 35-day-old plants of hd1-3 mutant and the wild-type Zhonghua11 grown under SD and LD conditions were collected. Total RNAs were extracted using TRIzol and mRNAs were purified for microarray analysis.
Contributor(s) Zhang S, Ma X, Luan W
Citation(s) 34357443
Submission date Feb 03, 2021
Last update date Aug 24, 2021
Contact name Xuan Ma
Organization name Tianjin Normal University
Department College of Life Sciences
Street address Binshui West Road
City Tianjin
ZIP/Postal code 300387
Country China
Platforms (1)
GPL2025 [Rice] Affymetrix Rice Genome Array
Samples (4)
GSM5060850 SD_WT
GSM5060851 SD_Mut
GSM5060852 LD_WT
BioProject PRJNA699148

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE166053_RAW.tar 20.3 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file

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