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Series GSE166118 Query DataSets for GSE166118
Status Public on Feb 04, 2021
Title Epigenetic Dysregulation by Polyglutamine-expanded Ataxin-7 In The mouse model of SCA7
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary To determine if STAGA complex dysfunction affects chromatin remodeling in SCA7 cerebellar degeneration, we pursued unbiased ChIP-Seq analysis of histone 3 lysine 9 acetylation (H3K9ac), as this histone mark is an established indicator of open chromatin in the transcription regulatory regions of most genes. We studied SCA7 266Q knock-in mice which faithfully recapitulate a severe, rapidly progressive neurodegenerative phenotype involving the cerebellum. We detected altered H3K9ac in the promoter regions of 777 genes in the cerebellum of SCA7 266Q knock-in mice and wild-type littermate control mice, and noted that the vast majority of these chromatin alterations corresponded to markedly increased H3K9 promoter acetylation in genes expressed in the cerebellum of SCA7 model mice.
 
Overall design Examination of H3K9ac histone modification in cerebella of symptomatic SCA7 266Q knock-in mice and wild type littermates.
 
Contributor(s) Delaney JR, Niu C, Switonski PM, La Spada AR
Citation(s) 34852229
Submission date Feb 03, 2021
Last update date Dec 08, 2021
Contact name Pawel Michal Switonski
Organization name University of California, Irvine
Department Department of Pathology & Laboratory Medicine
Lab La Spada
Street address 1001 Health Sciences Road
City Irvine
State/province California
ZIP/Postal code 92697
Country USA
 
Platforms (1)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
Samples (8)
GSM5061953 Cerebellum 266Q knock-in rep1
GSM5061954 Cerebellum 266Q knock-in rep2
GSM5061955 Cerebellum 266Q knock-in rep3
This SubSeries is part of SuperSeries:
GSE166123 Epigenetic dysregulation impairs high-fidelity DNA repair to promote cerebellar degeneration in spinocerebellar ataxia type 7
Relations
BioProject PRJNA699289
SRA SRP304588

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE166118_RAW.tar 991.5 Mb (http)(custom) TAR (of BED, BIGWIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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