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Series GSE166543 Query DataSets for GSE166543
Status Public on Feb 11, 2021
Title Ribosomes act as cryosensors in plants
Organism Arabidopsis thaliana
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary Cold temperatures are a threat to temperate plants, and Arabidopsis thaliana has acquired an adaptive gene expression network controlled by CBF transcription factors. The CBFs are sufficient to enable plants to survive otherwise lethal subzero temperatures. Constitutive CBF expression causes delayed flowering and stunted growth, and plants have evolved the ability to restrict CBF expression to occur only in the cold. This allows plants to anticipate likely freezing events and selectively deploy cold tolerance. The mechanism by which cold stress is sensed is however unknown. Here we show that protein translation rates in plants are proportional to temperature, and reduced translation rates trigger a rise in intracellular free calcium that activates the CAMTA transcription factors, and these directly activate cold-induced gene expression.
Overall design Single samples were taken at each time point. RNA-Seqs and ChIP-Seqs were performed for different genotypes at different temperature and objective time.
Contributor(s) Guillaume-Schöpfer D, Jaeger K, Geng F
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Submission date Feb 10, 2021
Last update date Feb 13, 2021
Contact name Philip Wigge
Organization name Cambridge University
Department Sainsbury Laboratory
Street address 47 Bateman street
City Cambridge
ZIP/Postal code CB2 1LR
Country United Kingdom
Platforms (1)
GPL19580 Illumina NextSeq 500 (Arabidopsis thaliana)
Samples (81)
GSM5074256 198CS1
GSM5074257 198CS10
GSM5074258 198CS11
BioProject PRJNA701247
SRA SRP305671

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE166543_RAW.tar 280.9 Mb (http)(custom) TAR (of BW, CSV, NARROWPEAK)
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Raw data are available in SRA
Processed data provided as supplementary file

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