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Series GSE166930 Query DataSets for GSE166930
Status Public on May 20, 2021
Title High-throughput sequencing of Lactobacillus casei BL23 sRNAs
Organism Lacticaseibacillus casei
Experiment type Expression profiling by high throughput sequencing
Summary In the present study we characterized the sRNA profile of Lactobacillus casei BL23 using conventional and mDOT-seq approaches. In total 307 potential sRNAs were identified, 28 of which were validated by Northern hybridization. As a major portion of predicted sRNAs were shown to be conserved among the related species of Lactobacillus casei group (LCG), the results obtained in this study could serve as a valuable resource for achieving a better understanding of LCG physiology or even improving their applications in industry and medicine.
Overall design Nine samples were prepared and analysed covering three different library preparations (two for mDOT-seq and one conventional) with three biological replicates each.
Contributor(s) Mickutė M, Kvederavičiūtė K, Vilkaitis G
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BioProject PRJNA678647
Submission date Feb 17, 2021
Last update date May 20, 2021
Contact name Kotryna Kvederaviciute
Organization name Vilnius University
Department Life Sciences Center
Lab Institute Of Biotechnology
Street address Sauletekio av. 7
City Vilnius
ZIP/Postal code 10257
Country Lithuania
Platforms (1)
GPL30169 Illumina NextSeq 500 (Lacticaseibacillus casei)
Samples (9)
GSM5088617 L. casei BL23_mDOT-seq U_replicate 1
GSM5088618 L. casei BL23_mDOT-seq U_replicate 2
GSM5088619 L. casei BL23_mDOT-seq U_replicate 3
This SubSeries is part of SuperSeries:
GSE166932 Methyltransferase-directed orthogonal tagging and sequencing of miRNAs and bacterial small RNAs in probiotic Lactobacillus casei
SRA SRP292855

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Supplementary file Size Download File type/resource
GSE166930_Casei_mir.tsv.gz 9.9 Kb (ftp)(http) TSV
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