|
| Status |
Public on May 20, 2021 |
| Title |
High-throughput sequencing of Lactobacillus casei BL23 sRNAs |
| Organism |
Lacticaseibacillus casei |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In the present study we characterized the sRNA profile of Lactobacillus casei BL23 using conventional and mDOT-seq approaches. In total 307 potential sRNAs were identified, 28 of which were validated by Northern hybridization. As a major portion of predicted sRNAs were shown to be conserved among the related species of Lactobacillus casei group (LCG), the results obtained in this study could serve as a valuable resource for achieving a better understanding of LCG physiology or even improving their applications in industry and medicine.
|
| |
|
| Overall design |
Nine samples were prepared and analysed covering three different library preparations (two for mDOT-seq and one conventional) with three biological replicates each.
|
| |
|
| Contributor(s) |
Mickutė M, Kvederavičiūtė K, Vilkaitis G |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| BioProject |
PRJNA678647 |
| |
| Submission date |
Feb 17, 2021 |
| Last update date |
May 20, 2021 |
| Contact name |
Kotryna Kvederaviciute |
| E-mail(s) |
kotryna.kvederaviciute@mif.vu.lt
|
| Organization name |
Vilnius University
|
| Department |
Life Sciences Center
|
| Lab |
Institute Of Biotechnology
|
| Street address |
Sauletekio av. 7
|
| City |
Vilnius |
| ZIP/Postal code |
10257 |
| Country |
Lithuania |
| |
|
| Platforms (1) |
| GPL30169 |
Illumina NextSeq 500 (Lacticaseibacillus casei) |
|
| Samples (9)
|
| GSM5088620 |
L. casei BL23_conventional RNA-seq_replicate 1 |
| GSM5088621 |
L. casei BL23_conventional RNA-seq_replicate 2 |
| GSM5088622 |
L. casei BL23_conventional RNA-seq_replicate 3 |
| GSM5088623 |
L. casei BL23_mDOT-seq C_replicate 1 |
| GSM5088624 |
L. casei BL23_mDOT-seq C_replicate 2 |
| GSM5088625 |
L. casei BL23_mDOT-seq C_replicate 3 |
|
| This SubSeries is part of SuperSeries: |
| GSE166932 |
Methyltransferase-directed orthogonal tagging and sequencing of miRNAs and bacterial small RNAs in probiotic Lactobacillus casei |
|
| Relations |
| SRA |
SRP292855 |
Less...
More...