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| Status |
Public on Feb 24, 2021 |
| Title |
Neural tube closure defect: Single Cell ATAC and single cell mRNA seq |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Single-cell mRNA sequencing was overlaid with single-cell ATAC sequencing of the mouse E9.5 cranial region for both wildtype and miR-302 knockout embryos, an established example of a neural tube closure defect. The goals of this study were to compare changes in gene expression and chromatin accessibility upon loss of a stem cell miRNA and neural tube closure defect.
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| Overall design |
The cranial region of E9.5 wildtype and miR-302 knockout embryos were dissociated and single cell/ single nuclei suspension was partitioned on a 10x chromium platform. One set of embryos was used for single cell mRNA and one set of embryos was used for single-cell ATAC sequencing
This Series represents scATAC-seq data.
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| Contributor(s) |
Keuls R |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Feb 24, 2021 |
| Last update date |
Feb 26, 2021 |
| Contact name |
Rachel Keuls |
| Organization name |
Baylor College of Medicine
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| Lab |
Ronald Parchem Lab
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| Street address |
One Baylor Plaza, Alkek N1020
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| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
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| Relations |
| BioProject |
PRJNA704678 |
| SRA |
SRP307980 |
