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Series GSE167468 Query DataSets for GSE167468
Status Public on Feb 25, 2021
Title Loss of Prdm12 during development, but not in mature nociceptors, causes defects in pain sensation.
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Prdm12 is a key transcription factor in nociceptor neurogenesis. Mutations of Prdm12 cause Congenital Insensitivity to Pain (CIP) due to failure of nociceptor development. However, precisely how deletion of Prdm12 during development or adulthood affects nociception is unknown. Here, we employ tissue- and temporal-specific knockout mouse models to test the function of Prdm12 during development and in adulthood. We find that constitutive loss of Prdm12 causes deficiencies in proliferation during sensory neurogenesis. We also demonstrate that conditional knockout from dorsal root ganglia (DRGs) during embryogenesis causes defects in nociception. In contrast, we find that in adult DRGs, Prdm12 is dispensable for most pain sensation and injury-induced hypersensitivity. Using transcriptomic analysis, we found mostly unique changes in adult Prdm12 knockout DRGs compared to embryonic knockout, and that PRDM12 is likely a transcriptional activator in the adult. Overall, we find that the function of PRDM12 changes over developmental time.
Overall design Bulk mRNA profiles of dorsal root ganglia (DRGs) from wild type (WT, n = 2) and Prdm12AvilERT2CKO (Mut, n = 2)
Contributor(s) Landy MA, Goyal M, Casey KM, Liu C, Lai HC
Citation(s) 33789102
Submission date Feb 24, 2021
Last update date Apr 20, 2021
Contact name Mark Landy
Organization name UT Southwestern Medical Center
Lab Lai Lab
Street address 5323 Harry Hines Blvd
City Dallas
State/province TX
ZIP/Postal code 75390
Country USA
Platforms (1)
GPL21626 NextSeq 550 (Mus musculus)
Samples (4)
GSM5105603 WT DRG Rep1
GSM5105604 WT DRG Rep2
GSM5105605 Mut DRG Rep 1
BioProject PRJNA704694
SRA SRP307995

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Supplementary file Size Download File type/resource
GSE167468_MUT_vs_WT_fpkm_matrix_whole_genome_fpkm_1.txt.gz 255.9 Kb (ftp)(http) TXT
GSE167468_Siggenes_WT_MUT_with_minFPKM_1_at_log2FC_1_FDR_0.05.txt.gz 6.1 Kb (ftp)(http) TXT
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