|
| Status |
Public on Feb 23, 2022 |
| Title |
Identifying novel surface markers for megakaryocyte progenitor cells [D27 transgene] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We carried out 10X sequencing of D27 induced pluripotent stem cell-derived megakaryocyte population which had been sorted for surface markers of megakaryocyte progenitors.
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| |
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| Overall design |
Two sets of transgene libraries, one overexpressing TAL1 and the other overexpressing GATA1 and FLI1, were generated from each cell, using primers specific for the ends of inserted lentiviral transgenes used to initiate differentiation.
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| |
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| Contributor(s) |
Lawrence M, Shahsavari A, Mohorianu I, Ghevaert C |
| Citation(s) |
35171685 |
| |
| Submission date |
Mar 04, 2021 |
| Last update date |
Feb 23, 2022 |
| Contact name |
Irina Mohorianu |
| E-mail(s) |
data-submissions@stemcells.cam.ac.uk
|
| Organization name |
University of Cambridge
|
| Department |
Wellcome-MRC Cambridge Stem Cell Institute
|
| Street address |
Puddicombe Way
|
| City |
Cambridge |
| ZIP/Postal code |
CB2 0AW |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (4)
|
| GSM5135779 |
D27 megakaryocyte culture sorted for progenitor cells, pWPRE.2 |
| GSM5135780 |
D27 megakaryocyte culture sorted for progenitor cells, TAL1.2 |
| GSM5135781 |
D27 megakaryocyte culture sorted for progenitor cells, pWPRE.1 |
| GSM5135782 |
D27 megakaryocyte culture sorted for progenitor cells, TAL1.1 |
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| This SubSeries is part of SuperSeries: |
| GSE168291 |
Identifying novel surface markers for megakaryocyte progenitor cells |
|
| Relations |
| BioProject |
PRJNA713842 |
| SRA |
SRP310314 |
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