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Series GSE168289 Query DataSets for GSE168289
Status Public on Feb 23, 2022
Title Identifying novel surface markers for megakaryocyte progenitor cells [D27 transgene]
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary We carried out 10X sequencing of D27 induced pluripotent stem cell-derived megakaryocyte population which had been sorted for surface markers of megakaryocyte progenitors.
Overall design Two sets of transgene libraries, one overexpressing TAL1 and the other overexpressing GATA1 and FLI1, were generated from each cell, using primers specific for the ends of inserted lentiviral transgenes used to initiate differentiation.
Contributor(s) Lawrence M, Shahsavari A, Mohorianu I, Ghevaert C
Citation(s) 35171685
Submission date Mar 04, 2021
Last update date Feb 23, 2022
Contact name Irina Mohorianu
Organization name University of Cambridge
Department Wellcome-MRC Cambridge Stem Cell Institute
Street address Puddicombe Way
City Cambridge
ZIP/Postal code CB2 0AW
Country United Kingdom
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (4)
GSM5135779 D27 megakaryocyte culture sorted for progenitor cells, pWPRE.2
GSM5135780 D27 megakaryocyte culture sorted for progenitor cells, TAL1.2
GSM5135781 D27 megakaryocyte culture sorted for progenitor cells, pWPRE.1
This SubSeries is part of SuperSeries:
GSE168291 Identifying novel surface markers for megakaryocyte progenitor cells
BioProject PRJNA713842
SRA SRP310314

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE168289_SIGAA6-barcodes.tsv.gz 2.4 Mb (ftp)(http) TSV
GSE168289_SIGAA6-features.tsv.gz 297.6 Kb (ftp)(http) TSV
GSE168289_SIGAB6-barcodes.tsv.gz 2.4 Mb (ftp)(http) TSV
GSE168289_SIGAB6-features.tsv.gz 297.6 Kb (ftp)(http) TSV
GSE168289_SIGAC6-barcodes.tsv.gz 2.4 Mb (ftp)(http) TSV
GSE168289_SIGAC6-features.tsv.gz 297.6 Kb (ftp)(http) TSV
GSE168289_SIGAD6-barcodes.tsv.gz 2.4 Mb (ftp)(http) TSV
GSE168289_SIGAD6-features.tsv.gz 297.6 Kb (ftp)(http) TSV
GSE168289_counts.mtx.gz 2.5 Mb (ftp)(http) MTX
GSE168289_meta.csv.gz 51.1 Kb (ftp)(http) CSV
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