Transcriptomic profiling of cancer patients from tumor tissue and organ-matched normal tissue as reference which were taken as part of the WINTHER clinical trial. The organ-matched normal tissues were used in order to eliminate host gene expression variability while discarding most genetic variability between individuals. The goal was to develop an algorithm, Digital Display Precision Predictor (DDPP), which aims to identify transcriptomic predictors of treatment outcome, which were recorded in the trial.
Overall design
Dual biopsies experiment - Tumor tissue vs. organ-matched normal tissue. 23 Samples were collected along with the outcomes under treatments, which was individualized based on the cancer type and individual status. Hybridizations were performed as dual-dye-swapped (direct and inversed labeling) experiments
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