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| Status |
Public on Mar 13, 2023 |
| Title |
Droplet-based single-cell RNA-sequencing facilitates trancritption analysis of small intestine CD4 cells from Germline free Xbp1ΔIEC and Xbp1fl/fl |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We performed theDroplet-based single-cell RNA-sequencing on small intestine CD4 cells from Germline free(GF) Xbp1ΔIEC and Xbp1fl/fl mice and found the effect of ER stress in epithelia cells on CD4 cells.
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| Overall design |
SI LP CD4 (CD45+CD4+TCRβ+) living cells from Germline free Xbp1ΔIEC and Xbp1fl/fl were FACS-sorted for sequencing. The sorted live cells were separated into a single droplet using the Chromium Single Cell 3' v3 kit according to the manufacturer’s instruction (10x Genomics). The libraries were sequenced on NovaSeq 6000 S2 platform.
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| Contributor(s) |
Duan J, Lambden C |
| Citation(s) |
36917985 |
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| Submission date |
Mar 15, 2021 |
| Last update date |
Jun 12, 2023 |
| Contact name |
jinzhi duan |
| E-mail(s) |
jduan2@bwh.harvard.edu
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| Phone |
6175257369
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| Organization name |
Brigham and Women' hospital
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| Street address |
6th Floor, Room 641, NRB, 77 Louis Pasteur Avenue
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| City |
Boston |
| State/province |
Massachusetts |
| ZIP/Postal code |
02115 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA714643 |
| SRA |
SRP310713 |
