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Series GSE169184 Query DataSets for GSE169184
Status Public on Feb 04, 2022
Title Uhrf1 control the proliferation and the differentiation of satellite cells [MBD-seq]
Organism Mus musculus
Experiment type Methylation profiling by high throughput sequencing
Summary DNA methylation is an essential epigenetic regulation for cellular identity. In muscle stem cells, termed satellite cells, DNA methylation patterns are dynamically changed during muscle regeneration. However, how these DNA methylation patterns are maintained remain unclear. Here, we demonstrate that a key epigenetic regulator Uhrf1 (ubiquitin-like with PHD and RING finger domains 1) is activated in proliferating but not expressed in quiescent or differentiated satellite cells. Ablation of Uhrf1 in satellite cell impairs the proliferation and differentiation of satellite cells, leading to failure of muscle regeneration. Loss of Uhrf1 in satellite cells alters transcriptional programs and leads to DNA hypomethylation with the activation of Cdkn1a and Notch signalling. Down-regulation of Cdkn1a and Notch signalling rescued the proliferation and differentiation defect in Uhrf1-deficient satellite cells. Therefore, this study suggest that Uhrf1 can regulate the self-renewal and differentiation of satellite cells through DNA methylation patterning.
Overall design MBD2-Seq of activated satellite cells in vitro from Control and Uhrf1-deficient mice in satellite cells.
Contributor(s) Imai Y, Sakai H, Sawada Y
Citation(s) 35243267
Submission date Mar 18, 2021
Last update date Mar 09, 2022
Contact name Hiroshi Sakai
Phone +81-89-960-5925
Organization name Ehime University
Department Proteo-Science Center
Lab Division of Integrative Pathophysiology
Street address Shitsukawa
City Toon
State/province Ehime
ZIP/Postal code 791-0295
Country Japan
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (4)
GSM5182587 OIL, methylated DNA
GSM5182588 TMX, methylated DNA
GSM5182589 OIL, non-methylated DNA
This SubSeries is part of SuperSeries:
GSE169193 Uhrf1 control the proliferation and the differentiation of satellite cells
BioProject PRJNA715492
SRA SRP311284

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Supplementary file Size Download File type/resource
GSE169184_tmx_vs_oil_q_e-4_peaks.txt.gz 1.1 Mb (ftp)(http) TXT
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