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Series GSE169193 Query DataSets for GSE169193
Status Public on Feb 04, 2022
Title Uhrf1 control the proliferation and the differentiation of satellite cells
Organism Mus musculus
Experiment type Methylation profiling by high throughput sequencing
Expression profiling by high throughput sequencing
Summary DNA methylation is an essential form of epigenetic regulation responsible for cellular identity. In muscle stem cells, termed satellite cells, DNA methylation patterns are tightly regulated during differentiation. However, it is unclear how these DNA methylation patterns affect the function of satellite cells. We demonstrate that a key epigenetic regulator, ubiquitin like with PHD and RING finger domains 1 (Uhrf1), is activated in proliferating myogenic cells but not expressed in quiescent satellite cells or differentiated myogenic cells in mice. Ablation of Uhrf1 in mouse satellite cells impairs their proliferation and differentiation, leading to failed muscle regeneration. Uhrf1-deficient myogenic cells exhibited aberrant up-regulation of transcripts, including Sox9, with the reduction of DNA methylation level of their promoter and enhancer region. These findings show that Uhrf1 is a critical epigenetic regulator of proliferation and differentiation in satellite cells, by controlling cell type-specific gene expression via maintenance of DNA methylation.
Overall design Refer to individual Series
Citation(s) 35243267
Submission date Mar 18, 2021
Last update date Mar 06, 2022
Contact name Hiroshi Sakai
Phone +81-89-960-5925
Organization name Ehime University
Department Proteo-Science Center
Lab Division of Integrative Pathophysiology
Street address Shitsukawa
City Toon
State/province Ehime
ZIP/Postal code 791-0295
Country Japan
Platforms (2)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (13)
GSM5182587 OIL, methylated DNA
GSM5182588 TMX, methylated DNA
GSM5182589 OIL, non-methylated DNA
This SuperSeries is composed of the following SubSeries:
GSE169184 Uhrf1 control the proliferation and the differentiation of satellite cells [MBD-seq]
GSE169192 Uhrf1 control the proliferation and the differentiation of satellite cells [RNA-Seq]
BioProject PRJNA715510

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