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Series GSE1693 Query DataSets for GSE1693
Status Public on Oct 18, 2004
Title A novel response to microtubule perturbation in meiosis
Organism Saccharomyces cerevisiae
Experiment type Expression profiling by array
Summary Cells were grown to saturation in YPD (YEP + 2% glucose) for 24 hours, diluted into YPA (YEP + 2% potassium acetate) at OD600= 0.3 and grown over night at 30C. Cells were washed with sterilized water the next day and re-suspended in SPII medium (0.3% potassium acetate, pH = 7.0) at OD600= 1.9 to induce sporulation. Cells were sporulated at room temperature or 30C as indicated. Sporulation medium containing benomyl was always prepared freshly on the day of the experiment following the directions in {Shonn, 2000 #90}. Briefly, DMSO (dimethyl sulfoxide, Sigma-Aldrich) or benomyl [Methyl 1-(butylcarbamoyl)-2-benzimidazolecarbamate, Sigma-Aldrich; 30 mg/ml stock in DMSO] was dissolved in near-boiling SPII medium to avoid precipitation. The medium was then allowed to slowly cool to 30C or room temperature. At the time of drug treatment, cells were filtered and immediately re-suspended in the medium containing benomyl or DMSO.
Keywords = benomyl sporulation saccharomyces microtubule spindle checkpoint
Keywords: ordered
 
Web link http://www.biozentrum.unibas.ch/personal/primig/benomyl/
http://www.biozentrum.unibas.ch/primig/benomyl/
 
Contributor(s) Hochwagen A, Wrobel G, Cartron M, Demougin P, Wiederkehr C, Boselli M, Primig M, Amon A
Citation(s) 15899877
Submission date Aug 20, 2004
Last update date Jul 01, 2016
Contact name Michael Primig
E-mail(s) michael.primig@unibas.ch
Phone +41 61 267 2098
Fax +41 61 267 3398
URL http://www.biozentrum.unibas.ch/primig/
Organization name Biozentrum and Swiss Institute of Bioinformatics
Department Genome Bioinformatics
Lab Primig
Street address Klingelbergstr 50-70
City Basel
State/province Basel Stadt
ZIP/Postal code 4056
Country Switzerland
 
Platforms (1)
GPL90 [YG_S98] Affymetrix Yeast Genome S98 Array
Samples (26)
GSM29073 SD8 - Replicate 1
GSM29077 SD8 - Replicate 2
GSM29080 SD5 - Replicate 1
Relations
BioProject PRJNA90655

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE1693_RAW.tar 47.0 Mb (http)(custom) TAR (of CEL, EXP, RPT)

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