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Series GSE16936 Query DataSets for GSE16936
Status Public on Dec 05, 2009
Title AMD3100 and G-CSF Mobilize Different CD34+ Cell Populations Based on Gene and miRNA Expression
Platform organisms Homo sapiens; Mus musculus; Rattus norvegicus; human gammaherpesvirus 4; Human gammaherpesvirus 8; Betapolyomavirus macacae
Sample organisms Macaca mulatta; Homo sapiens
Experiment type Expression profiling by array
Non-coding RNA profiling by array
Summary Plerixafor (AMD3100) and G-CSF mobilize peripheral blood stem cells (PBSCs) by different mechanisms. A rhesus macaque model was used to compare plerixafor and G-CSF-mobilized CD34+ cells. Three PBSC concentrates were collected from 3 macaques treated with G-CSF, plerixafor, or plerixafor plus G-CSF. CD34+ cells were isolated by immunoselection and were analyzed by global gene and micro RNA (miR) expression microarrays. Unsupervised hierarchical clustering of the gene expression data separated the CD34+ cells into three groups based on mobilization regimen. Plerixafor-mobilized cells were enriched for B cells, T cells and mast cells genes and G-CSF-mobilized cells were enriched for neutrophils, and mononuclear phagocytes (MPs) genes. Genes up-regulated in plerixafor plus G-CSF-mobilized CD34+ cells included many that were not up-regulated by either agent alone. Two hematopoietic progenitor cell miR, miR-10 and miR-126, and a dendritic cell miR, miR-155, were up-regulated in G-CSF-mobilized CD34+ cells. A pre-B cell acute lymphocytic leukemia miR, miR-143-3p, and a T cell miR, miR-143-5p, were up-regulated in plerixafor plus G-CSF-mobilized cells. The composition of CD34+ cells is dependent on the mobilization protocol. Plerixafor-mobilized CD34+ cells include more B, T, and mast cell precursors while G-CSF-mobilized cells have more neutrophil and MP precursors.
Overall design Three rhesus macaques were given all three mobilization protocols and PBSCs were collected from each of these three animals. Two monkeys were given plerixafor first, one was given G-CSF first and all three were given plerixafor plus G-CSF last. This resulted in 6 samples (CD34+ and CD34- cells from each of the 3 mobilizations) from each of the 3 individual animals (biological replicates).
Citation(s) 19602709
Submission date Jul 02, 2009
Last update date Jan 18, 2013
Contact name Francesco Maria Marincola
Phone 301-793-8210
Organization name Sidra Medical and Research Center
Street address Al Nasr Tower, AL Corniche Street, PO Box 26999
City Doha
ZIP/Postal code PO Box 26999
Country Qatar
Platforms (2)
GPL8793 CCDTM Homo sapiens 17.5k version 2.1
GPL8794 CCDTM miRNA 850 version 4.1
Samples (36)
GSM424551 Rh1 - CD34+ -AMD3100 - mAdbID:103241
GSM424552 Rh1 - CD34- AMD3100 - mAdbID:103242
GSM424553 Rh3 - CD34+ -AMD3100 - mAdbID:103243
BioProject PRJNA117649

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Supplementary file Size Download File type/resource
GSE16936_RAW.tar 35.5 Mb (http)(custom) TAR (of GPR)
Processed data included within Sample table

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