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Series GSE169433 Query DataSets for GSE169433
Status Public on Apr 01, 2022
Title The Arabidopsis F-box protein FBW2 degrades AGO1 to avoid spurious loading of illegitimate small RNA [PARE-seq]
Organism Arabidopsis thaliana
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary RNA silencing is a conserved mechanism in eukaryotes and is involved in development, heterochromatin maintenance and defense against viruses. In plants, ARGONAUTE1 (AGO1) protein plays a central role in both microRNA (miRNA) and small interfering RNA (siRNA)-directed silencing and its expression is regulated at multiple levels. Here we report that the F-box protein FBW2 targets proteolysis of AGO1 by a CDC48-mediated autophagy mechanism. We found that FBW2 assembles an SCF complex that recognizes the MID-PIWI domain of AGO1 and requires its C-terminal domain containing a GW motif for AGO1 turnover. We show that FBW2 has a preference for unloaded AGO1 and also for some mutated forms of the protein. FBW2 loss of function increases RNA silencing activity, but does not lead to strong growth or developmental defects. However, under conditions in which small RNA production or accumulation is affected, the failure to degrade AGO1 in fbw2 mutants becomes more deleterious for the plant. Hence, we show that the non-degradable AGO1 protein assembles high molecular complexes and binds illegitimate small RNAs.
Overall design PARE seq profiling of five different Arabidopsis mutants and the corresponding wildtype (Col0) with 3 biological replicates each from Total RNA.
Web link
Contributor(s) Baldrich P, Hacquard T, Clavel M, Lechner E, Pérez-Salamó I, Schepetilnikov M, Derrien B, Dubois M, Baumberger N, Vaucheret H, Meyers BC, Genschik P
Citation(s) 35417704
Submission date Mar 23, 2021
Last update date Jun 01, 2022
Contact name Patricia Baldrich
Organization name Donald Danforth Plant Science Center
Department Meyers Lab
Lab Meyers Lab
Street address 975 N warson Road
City Saint Louis
State/province MO
ZIP/Postal code 63132
Country USA
Platforms (1)
GPL17639 Illumina HiSeq 2500 (Arabidopsis thaliana)
Samples (18)
GSM5203023 Col0_1
GSM5203024 fbw2_1
GSM5203025 hyl1_1
This SubSeries is part of SuperSeries:
GSE169434 The Arabidopsis F-box protein FBW2 degrades AGO1 to avoid spurious loading of illegitimate small RNA
BioProject PRJNA716605
SRA SRP311815

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Supplementary file Size Download File type/resource
GSE169433_RAW.tar 975.0 Mb (http)(custom) TAR (of TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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