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Series GSE17010 Query DataSets for GSE17010
Status Public on Apr 22, 2010
Title Differential pre-mRNA defects in fission yeast carrying a temperature degron allele of the SMN gene
Organism Schizosaccharomyces pombe
Experiment type Expression profiling by genome tiling array
Summary The SMA disease results from deletions or mutations in the “survival of motor neuron” (SMN1) gene. The SMN protein plays an essential role in the biogenesis of spliceosomal snRNPs but the link between a defect in this process and specific splicing inhibition is not known. Here, we report the construction of a temperature degron (td) allele of the S. pombe SMN protein and show that its depletion at 37°C affects snRNPs stability and splicing. In addition, the function of the tdSMN allele in snRNPs assembly is affected already at 25°C suggesting a deleterious effect of the tag at this temperature. Remarkably, using S. pombe tiling arrays and RT-PCR validation tests, we report that splicing is altered differentially in the tdSMN cells at permissive temperature. Our results suggest that the defects observed in tdSMN fission yeast cells mimic splicing deficits observed in SMN-deficient metazoan cells.
Overall design Total RNA (triplicate experiment) from the td-SMN strain grown at 25°c vs total RNA from the wild type strain (triplicate experiment)
Contributor(s) Campion Y, Neel H, Gostan T, Soret J, Bordonné R
Citation(s) 20400941
Submission date Jul 08, 2009
Last update date May 27, 2014
Contact name johann Soret
Organization name CNRS
Department Institut de Genetique Moleculaire
Lab Assembly and traffic of ribonucleoproteins
Street address 1919, route de Mende
City Montpellier
ZIP/Postal code 34293
Country France
Platforms (1)
GPL7715 [Sp20b_M] Affymetrix S. pombe Tiling 1.0FR Array
Samples (1)
GSM425513 3td-SMN vs 3WT
BioProject PRJNA117751

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE17010_RAW.tar 78.3 Mb (http)(custom) TAR (of BAR, CEL, TXT)
Processed data provided as supplementary file

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